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Removal of 5- and 3-untranslated regions enhances erythropoietin gene expression in mammalian cells

Removal of 5- and 3-untranslated regions enhances erythropoietin gene expression in mammalian cells

Molecular Biology 35(3): 0-3

ISSN/ISBN: 0026-8933

DOI: 10.1023/a:1010466526838

The effect of the 5"- and 3"-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5"-UTR-deleted, 3"-UTR-deleted, and 5",3"-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5",3"-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5",3"-UTR-deleted gEpo might be related with the transcription efficiency. Reprinted by permission of the publisher.

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Accession: 018093243

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