EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

Role of the anomalous rectifier in determining membrane potentials of mouse muscle fibres at low extracellular K+


Role of the anomalous rectifier in determining membrane potentials of mouse muscle fibres at low extracellular K+



Journal of Physiology 434: 549-560



ISSN/ISBN: 0022-3751

PMID: 2023129

DOI: 10.1113/jphysiol.1991.sp018485

1. The membrane potential (Vm) of fibres of the extensor digitorum longus (EDL) of the mouse, measured at 35 degrees C and with extracellular potassium concentration (K+o) 5.7 mM, was Vm = -76 mV. 2. Lowering K+o below 1 mM could lead to either a hyperpolarizing or a depolarizing response. When Vm was lower than -75.5 mV in the control medium, a reduction of K+o to 0.76 mM led to a hyperpolarization of Vm (-95.0 +/- 0.7 mV, n = 40); otherwise a depolarization occurred (Vm = -47.2 +/- 1.1 mV, n = 21). 3. The difference in Vm responses did not correlate consistently with functional differences in cell types, as cells that originally hyperpolarized, could later depolarize. 4. The observed phenomena could be explained if the properties of the anomalous rectifier, AR (or inward-going rectifier), are considered to be similar to those observed in cardiac cells. 5. Apparently caesium acted as a competitive inhibitor; when the inhibition was strong enough the non-linear properties of the AR regeneratively amplified the depolarization to the full-blown depolarized state (Vm = -46.7 +/- 1.3 mV, n = 15). 6. Ouabain (10(-4) M) reduced Vm (to -45 +/- 3 mV, n = 5) and reduced dramatically the selectivity of the cell membrane for potassium over sodium. These effects could be reversed readily by washing out the ouabain. 7. Adrenaline (2 microM) added to the medium hyperpolarized Vm (delta Vm = -4.6 +/- 1.4 mV, n = 9) and increased the changes induced by lowered K+o (from -14.3 +/- 0.5 mV, n = 5 to -18.0 +/- 0.8 mV, n = 9); the cells that originally depolarized when K+o was lowered could hyperpolarize after adrenaline addition.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018102979

Download citation: RISBibTeXText

Related references

The anomalous rectifier in isolated mouse muscle fibers with low extracellular potassium. Journal of Physiology (Cambridge) 420: 94P, 1990

Computed extracellular potentials of a number of muscle fibres. II. Communication. Effect of axial displacement of the muscle fibres. Electromyography and Clinical Neurophysiology 18(6): 517-526, 1978

The resting membrane potentials of fast and slow skeletal muscle fibres in the developing mouse. Comparative Biochemistry and Physiology 33(4): 923-931, 1970

Computed extracellular potentials of a number of muscle fibres. I. Communication. Influence of the desynchronization in the fibres activation. Electromyography and Clinical Neurophysiology 18(5): 361-376, 1978

Estimating the duration of intracellular action potentials in muscle fibres from single-fibre extracellular potentials. Journal of Neuroscience Methods 197(2): 221-230, 2011

Effect of temperature on the inward rectifier and gramicidin A-induced channels in the membrane of frog skeletal muscle fibres. General Physiology and Biophysics 5(1): 47-51, 1986

Detubulation experiments localise delayed rectifier currents to the surface membrane of amphibian skeletal muscle fibres. Journal of Muscle Research and Cell Motility 25(4-5): 389-395, 2004

The role of potassium and sodium ions in the development of membrane potentials of muscle fibres at a lowered intracellular potassium concentration. Tsitologiia 8(6): 750-755, 1966

Chlorpromazine haloperidol and bepridil differentially block inward rectifier and delayed outward potassium currents in mouse skeletal muscle fibres. Japanese Journal of Pharmacology 59(Suppl. 1): 266P, 1992

Influence of the fibre end on the calculated extracellular potentials of single muscle fibres. Electromyography and Clinical Neurophysiology 25(6): 443-451, 1985

On the deduction of bioelectric potentials of single skeletal muscle fibres by sucrose gap method. II. On additionally induced potentials due to the action of potassium chloride and acid on the membrane. Acta Biologica et Medica Germanica 21(1): 67-77, 1968

Dependence between intra- and extracellular action potentials of isolated frog muscle fibres at different temperatures. Acta Physiologica et Pharmacologica Bulgarica 14(4): 12-19, 1988

Generation of membrane potentials in lepidopteran muscle I. Analysis of a mixed electrode system in the skeletal muscle fibres of Sphinx ligustri. Arch Int Physiol Biochim 75(2): 245-260, 1967

Generation of Membrane Potentials in Lepidopteran Muscle: I. Analysis of a Mixed Electrode System in the Skeletal Muscle Fibres of Sphinx Ligustbi (L.). Archives Of Physiology And Biochemistry 75(2): 245-260, 1967

Generation of membrane potentials in lepidopteran muscle. I. Analysis of a mixed electrode system in the skeletal muscle fibres of Sphinx ligustri (L.). Archives Internationales de Physiologie et de Biochimie 75(2): 245-260, 1967