+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Secretory protein expression patterns during rat parotid gland development

Secretory protein expression patterns during rat parotid gland development

Anatomical Record 252(3): 485-497

ISSN/ISBN: 0003-276X

PMID: 9811227

DOI: 10.1002/(sici)1097-0185(199811)252:3<485::aid-ar17>;2-j

The rat parotid gland produces a number of well-characterized secretory proteins. Relatively little is known, however, about the onset of their synthesis and cellular localization during gland development. Secretory protein expression was studied in parotid glands of fetal and postnatal rats using light and electron microscopic immunocytochemistry and Northern blotting. Amylase, parotid secretory protein (PSP), common salivary protein-1 (CSP-1), and SMGB were first detected by immunofluorescence in parotid glands of 18 day fetuses. By 5 days after birth, light and electron microscopic immunolabeling localized all of these proteins to the secretory granules of developing acinar cells. Labeling of acinar cells for DNAse I, however, was not observed until 18 days after birth. Between 9 and 25 days, CSP-1 and SMGB reactivity of acinar cells declined, but increased in intercalated duct cells. After 25 days, CSP-1 and SMGB were found only m intercalated ducts, and amylase, PSP, and DNAse I were restricted to acinar cells. Levels of CSP-1 and SMGB mRNA were relatively constant through 21 postnatal days, but declined significantly after that. Amylase and PSP mRNA increased rapidly and continuously from five days after birth to the adult stage. In contrast, DNAse I mRNA was not detectable until 18 days after birth. The immunocytochemical and molecular analyses define three basic patterns of proteins expression in the rat parotid gland: proteins whose synthesis is initiated early in development and is maintained in the acinar cells, such as amylase and PSP; proteins that are initially synthesized by immature acinar cells but are restricted to intercalated ducts in the adult gland, such as CSP-1 and SMGB; and proteins that are synthesized only by mature acinar cells and first appear during the third postnatal week, such as DNAse I. The parotid gland exhibits four distinct developmental stages: prenatal, from initiation of the gland rudiment until birth; neonatal, from 1 day up to about 9 days postnatal; transitional, from 9 days to 25 days of age; and adult, from 25 days on. Although differences exist in timing and in the specific proteins expressed, these developmental stages are similar to those seen in the rat submandibular gland. Additionally, the results support the suggestion that intercalated ducts may differentiate from the neonatal acini.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 018108779

Download citation: RISBibTeXText

Related references

Protein-binding elements in the proximal parotid secretory protein gene enhancer essential for salivary-gland-specific expression. Biochemical Journal 357(Pt 2): 537-544, 2001

Development of secretory function in rat parotid gland. Pediatric Research 12(2): 100-104, 1978

Neonatal rat submandibular gland protein SMG-A and parotid secretory protein are alternatively regulated members of a salivary protein multigene family. Journal of Biological Chemistry 267(4): 2679-2687, 1992

Concomitant synthesis of membrane protein and exportable protein of the secretory granule in rat parotid gland. Journal of Cell Biology 50(1): 187-200, 1971

Concomitant Synthesis Of Membrane Protein And Exportable Protein Of The Secretory Granule In Rat Parotid Gland. The Journal of Cell Biology 50(1): 187-200, 1971

Reciprocal expression of c-jun, proline-rich protein and amylase genes during rat parotid salivary gland development. Differentiation; Research in Biological Diversity 51(3): 225-232, 1992

Secretory proline rich proteins appear early in parotid gland development. Journal of Dental Research 66(SPEC ISSUE MAR): 305, 1987

Acidic epididymal glycoprotein is a major secretory protein of rat parotid gland. Critical Reviews in Oral Biology and Medicine 4(3-4): 617, 1993

Latent stimulation of secretory protein synthesis in the rat parotid gland following isoproterenol. Journal of Cell Biology 55(2 PT 2): 155A, 1972

High levels of GM(1)-ganglioside and GM(1)-ganglioside beta-galactosidase in the parotid gland: a new model for secretory mechanisms of the parotid gland. Otolaryngologic Clinics of North America 32(5): 779-791, 1999

The purification and partial characterization of phospholipase A2, a secretory protein of rabbit parotid gland. Biochimica et Biophysica Acta 666(2): 259-274, 1981

Latent stimulation of secretory protein synthesis in the rat parotid gland following isoproterenol administration. Anatomical Record 175(2): 371, 1973

Protein biosynthesis in a cell-free system of the bovine parotid gland. 1. Incorporation in vitro of glycine-2-C14 into protein by parotid gland microsomes. Journal of Nihon University School of Dentistry 9(1): 24-30, 1967

Calcium-dependent protein kinase reactions associated with parotid gland secretory granule membranes. Journal of Dental Research 66(2): 557-563, 1987

Protein, IgA, secretory IgA, IgG and electrolytes as parameters of parotid gland function in children with cystic fibrosis. Monographs in Paediatrics 10: 135-137, 1979