+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Similarities and differences between human and murine TNF promoters in their response to lipopolysaccharide

Similarities and differences between human and murine TNF promoters in their response to lipopolysaccharide

Journal of Immunology 162(7): 4045-4052

ISSN/ISBN: 0022-1767

PMID: 10201927

Transcription of the TNF gene is rapidly and transiently induced by LPS in cells of monocyte/macrophage lineage. Previous data suggested that multiple NF-kappaB/Rel binding sites play a role in the transcriptional response to LPS of the murine gene. However, the relevance of homologous sites in the human TNF gene remained a matter of controversy, partly because the high affinity NF-kappaB/Rel site located at -510 in the murine promoter is not conserved in humans. Here we used two sets of similarly designed human and mouse TNF promoter deletion constructs and overexpression of IkappaB in the murine macrophage cell line ANA-1 to show remarkable similarity in the pattern of the transcriptional response to LPS, further demonstrating the functional role of the distal promoter region located between -600 and -650. This region was characterized by mutagenesis of protein binding sites, including two relatively low affinity NF-kappaB/Rel sites, #2 and 2a. Mutation in each of the NF-kappaB sites resulted in 2- to 3-fold lower transcriptional activity in response to LPS. In contrast to LPS activation, the response to PMA was substantially lower in magnitude and required only the proximal promoter region. In summary, the functional topography of human and murine promoters when assayed in the same system has some marked similarities. Our observations support the notion that full LPS response of TNF gene requires both NF-kappaB and non-NF-kappaB nuclear proteins. Our data also suggest that the functional activity of a given kappaB site depends on the entire DNA sequence context in the promoter region.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 018116078

Download citation: RISBibTeXText

Related references

Transcriptional regulation of the murine Presenilin-2 gene reveals similarities and differences to its human orthologue. Gene 446(2): 81-89, 2009

The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its human counterpart. Gene 158(2): 253-256, 1995

Structural properties of promoters: similarities and differences between prokaryotes and eukaryotes. Nucleic Acids Research 33(10): 3165-3175, 2005

Similarities and differences between the subgenomic and minus-strand promoters of an RNA plant virus. Journal of Virology 78(8): 4048-4053, 2004

Similarities and differences in the conformation of protein-DNA complexes at the U1 and U6 snRNA gene promoters. Nucleic Acids Research 28(14): 2771-2778, 2000

Spatio-temporal differences in the profile of murine brain expression of proinflammatory cytokines and indoleamine 2,3-dioxygenase in response to peripheral lipopolysaccharide administration. Journal of Neuroimmunology 200(1-2): 90-99, 2008

Human exposure at two radio frequencies (450 and 2450 MHz): Similarities and differences in physiological response. Bioelectromagnetics Suppl. 4: 12-20, 1999

Similarities and differences of X and Y chromosome homologous genes, SRY and SOX3, in regulating the renin-angiotensin system promoters. Physiological Genomics 47(5): 177-186, 2015

Molecular Basis of the Functional Differences between Soluble Human Versus Murine MD-2: Role of Val135 in Transfer of Lipopolysaccharide from CD14 to MD-2. Journal of Immunology 196(5): 2309-2318, 2016

DNA sequence and structure properties analysis reveals similarities and differences to promoters of stress responsive genes in Arabidopsis thaliana. International Journal of Data Mining and Bioinformatics 13(1): 1-12, 2015

Exploring the translational disconnect between the murine and human inflammatory response: analysis of LPS dose-response relationship in murine versus human cell lines and implications for translation into murine models of sepsis. Journal of Inflammation Research 8: 201-209, 2015

Bacterial lipopolysaccharide-mediated fetal death. Production of a newly recognized form of inducible cyclooxygenase (COX-2) in murine decidua in response to lipopolysaccharide. Journal of Clinical Investigation 95(2): 725-731, 1995

A murine model of HUS: Shiga toxin with lipopolysaccharide mimics the renal damage and physiologic response of human disease. Journal of the American Society of Nephrology: Jasn 17(12): 3404-3414, 2006

Human FMR1 and hnRNP-A2 promoters share some structural and functional similarities. American Journal of Human Genetics 61(4 Suppl. ): A166, 1997

Similarities and differences between selective and nonselective BAFF blockade in murine SLE. Journal of Clinical Investigation 116(3): 724-734, 2006