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Stabilization of recA protein-ssDNA complexes by the single-stranded DNA binding protein of Escherichia coli



Stabilization of recA protein-ssDNA complexes by the single-stranded DNA binding protein of Escherichia coli



Biochemistry 29(3): 837-843



In vitro recombination reactions promoted by the recA protein of Escherichia coli are enhanced by the single-stranded DNA binding protein (SSB). SSB affects the assembly of the filamentous complexes between recA protein and ssDNA that are the active form of the recA protein. Here, we present evidence that SSB plays a complex role in maintaining the stability and activity of recA-ssDNA filaments. Results of ATPase, nuclease protection, and DNA strand exchange assays suggest that the continuous presence of SSB is required to maintain the stability of recA-ssDNA complexes under reaction conditions that support their recombination activity. We also report data that indicate that there is a functional distinction between the species of SSB present at 10 mM magnesium chloride, which enhances recA-ssDNA binding, and a species present at 1 mM magnesium chloride, which displaces recA protein from ssDNA. These results are discussed in the context of current models of SSB conformation and of SSB action in recombination activities of the recA protein.

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Accession: 018126216

Download citation: RISBibTeXText

PMID: 2186808

DOI: 10.1021/bi00455a034


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