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The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription termination

Kainz, M.; Gourse, R.L.

Journal of Molecular Biology 284(5): 1379-1390

1999

ISSN/ISBN: 0022-2836
PMID: 9878357
DOI: 10.1006/jmbi.1998.2272
Accession: 018149454
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We screened a collection of single alanine residue substitution mutants spanning the entire C-terminal domain of the alpha subunit (alphaCTD) of Escherichia coli RNA polymerase (RNAP) for defects in rho-dependent transcription termination at lambdatR1 in vivo and in vitro, and thereby identified a patch of amino acid residues in the alphaCTD required for efficient rho-dependent termination. NusA addition led to the stimulation of rho-dependent termination under our conditions in vitro.

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Related References

Kainz, M.; Gourse, R.L. 1996: The C-terminal domain of the alpha subunit of E coli RNA polymerase is required for efficient Rho-dependent transcription termination Abstracts of the General Meeting of the American Society for Microbiology 96: 524
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