The activity of human kidney arylsulfatases A and B towards catecholamine sulfates
The activity of human kidney arylsulfatases A and B towards catecholamine sulfates
Demassieux, S.; Romain, Y.; Bordeleau, L.; Gravel, D.; Carrière, S.
Biochemistry and Cell Biology 64(10): 1044-1048
1986
ISSN/ISBN: 0829-8211
PMID: 3467755
DOI: 10.1139/o86-138
Both arylsulfatases (EC 3.1.6.1, ARS) A and B purified from human kidney displayed Michaelis-Menten kinetics towards catecholamines sulfates as substrates with Km values in the range of 4-25 mM. ARS A hydrolyzed adrenaline 3-sulfate and noradrenaline 3-sulfate with a maximal rate lower than that observed for cerebroside 3-sulfate. In contrast, ARS B hydrolyzed adrenaline 3-sulfate and noradrenaline 3-sulfate with a maximal rate similar to that observed for UDP-N-acetylgalactosamine 4-sulfate.