EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

The alloreactive and self-restricted CD4+ T cell response directed against a single MHC class II/peptide combination


The alloreactive and self-restricted CD4+ T cell response directed against a single MHC class II/peptide combination



Journal of Immunology 165(3): 1285-1293



ISSN/ISBN: 0022-1767

PMID: 10903728

DOI: 10.4049/jimmunol.165.3.1285

The cellular basis for allograft rejection derives from the strong T cell response to cells bearing foreign MHC. While it was originally assumed that alloreactive T cells focus their recognition on the polymorphic residues that differ between syngeneic and allogeneic MHC molecules, studies with MHC class I-restricted CTL have shown that MHC-bound peptides play a critical role in allorecognition. It has been suggested that alloreactive T cells depend more strongly on interactions with the MHC molecule than with the associated peptide, but there is little evidence to support this idea. Here we have studied the alloreactive and self-restricted response directed against the class II H2-Ab molecule bound with a single peptide, Ep, derived from the H2-Ealpha chain. This MHC class II-peptide combination was a poor target and stimulator of alloreactive CD4+ T cell responses, indicating that MHC-bound peptides are as important for alloreactive CD4+ T cells as they are for alloreactive CTL. We also generated alloreactive T cells with exquisite specificity for the Ab/Ep complex, and compared their reactivity with self-restricted T cells specific for the same Ab/Ep complex. Our results showed that peptide-specific alloreactive T cells, as compared with self-restricted T cells, were more sensitive to peptide stimulation, but equally sensitive to amino acid substitutions in the peptide. These findings indicate that alloreactive and self-restricted T cells interact similarly with their MHC/peptide ligand.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018159956

Download citation: RISBibTeXText

Related references

Dual HLA class I and class II restricted recognition of alloreactive T lymphocytes mediated by a single T cell receptor complex. Proceedings of the National Academy of Sciences of the United States of America 98(12): 6806-6811, 2001

Analysis of the requirements for class II-restricted T cell recognition of a single determinant reveals considerable diversity in the T cell response and degeneracy of peptide binding to I-Ed. Journal of Immunology 155(6): 2849-2857, 1995

Mhc restriction mechanisms of immune response in human neurocysticercosis. Human Immunology 23(2): 153-154, 1988

Peptide specificity of alloreactive CD4 positive T lymphocytes directed against a major histocompatibility complex class I disparity. Transplantation 62(10): 1485-1491, 1996

Existence of MHC class I-restricted alloreactive CD4+ T cells reacting with peptide transporter-deficient cells. Immunogenetics 53(8): 626-633, 2001

Beta-amino acid scan of a class I major histocompatibility complex-restricted alloreactive T-cell epitope. Journal of Biological Chemistry 276(27): 24525-24530, 2001

Requirement for peptide in alloreactive CD4+ T cell recognition of class II MHC molecules. Journal of Immunology 154(10): 5153-5164, 1995

Flow cytometric enumeration of Class I HLA-restricted, peptide-specific CD8+ T lymphocytes using tetramer technology and single-platform absolute T-cell counting. Journal of Biological Regulators and Homeostatic Agents 17(3): 268-278, 2003

The natural immune response to inhaled soluble protein antigens involves major histocompatibility complex (MHC) class I-restricted CD8+ T cell-mediated but MHC class II-restricted CD4+ T cell-dependent immune deviation resulting in selective suppression of immunoglobulin E production. Journal of Experimental Medicine 178(3): 889-899, 1993

Alloreactive cytolytic T cell clones with dual recognition of HLA-B27 and HLA-DR2 antigens. Selective involvement of CD8 in their class I--directed cytotoxicity. Journal of Experimental Medicine 165(2): 428-443, 1987

HLA class I-restricted lysis of leukemia cells by a CD8+ cytotoxic T-lymphocyte clone directed against WT1 peptide. Blood 92(10 Suppl. 1 Part 1-2): 616A, 1998

The H-2Db-restricted T cell response to a single viral peptide confers resistance to Theilers virus. FASEB Journal 16(4): A307, 2002

Frequency of HLa-A*0201-restricted cytotoxic T cell precursors and effectors directed to a single peptide of melan-A/mart-1 melanoma antigen Evidence for heterogeneity among melanoma patients. Proceedings of the American Association For Cancer Research Annual Meeting 38: 616, 1997

Overlapping epitopes that are recognized by CD8+ HLA class I-restricted and CD4+ class II-restricted cytotoxic T lymphocytes are contained within an influenza nucleoprotein peptide. Journal of Immunology 148(3): 894-899, 1992

IL-12-Dependent enhancement of CTL response to weak class I-restricted peptide immunogens requires coimmunization with T helper cell immunogens. Clinical Immunology 94(3): 200-211, 2000