+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

The biosynthetic pathway of pyrimidine (deoxy)nucleotides: a sensor of oxygen tension necessary for maintaining cell proliferation?

The biosynthetic pathway of pyrimidine (deoxy)nucleotides: a sensor of oxygen tension necessary for maintaining cell proliferation?

Experimental Cell Research 182(2): 673-680

ISSN/ISBN: 0014-4827

PMID: 2721599

DOI: 10.1016/0014-4827(89)90271-1

Culture experiments on Ehrlich ascites tumor cells revealed that a low oxygen tension (about 20% in normoxic atmosphere) induced an increase in the length of the growth cycle. The relative growth of aerobic control cells after transfer to the second in vitro passage was 145% within 24 h, and reduced to 50% at 1% O2 and about 30% at 0.1% O2. The increase in protein and DNA content of these hypoxic cultures was equally impaired. Also, the cell cycle traverse as analyzed by flow cytometry was affected predominantly at the GI/early S stage. Uptake of labeled thymidine into acid-insoluble material of hypoxic cells was below that of controls whereas incorporation of uridine exceeded that of normoxic controls. Supplementation of cells cultured under 0.1 and 1% O2 with 0.1 mM uridine or 0.1 mM deoxycytidine + 0.01 mM deoxyadenosine and deoxyguanosine improved all growth parameters; deoxynucleosides were more effective than uridine in cells under 0.1% O2 whereas in cells cultured under 1% O2 similar effects of both were observed. This points to an insufficient supply of nucleic acid precursors even under moderate limitations of oxygen tension and not only under strict hypoxia. Whereas a 12-h cultivation time at 0.1% O2 hardly impaired cell growth after reoxygenation, a cultivation time of 24 h considerably reduced the cellular capability to recover. This was alleviated by addition of (deoxy)nucleosides from the beginning of hypoxic culture. The results are interpreted as supporting the concept that the biosynthetic pathway of pyrimidine (deoxy)nucleotides.sbd.because of two oxygen-dependent enzymes, dihydroorotate dehydrogenase and ribonucleotide a potential transducer of environmental limitations in oxygen tension to the proliferative capacity of cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 018161498

Download citation: RISBibTeXText

Related references

The biosynthetic pathway of pyrimidine deoxynucleotides a potential transducer of environmental limitations in oxygen tension to cell cycle progression. European Journal of Cell Biology Suppl. (26): 40, 1989

Regulation of cell proliferation under extreme and moderate hypoxia: the role of pyrimidine (deoxy)nucleotides. British Journal of Cancer 70(5): 857-866, 1994

Synthesis and study of 5 poly fluoroalkyl 2' deoxy pyrimidine and 5 poly fluoroalkoxymethyl 2' deoxy pyrimidine nucleotides. Soviet Journal of Bioorganic Chemistry 5(1): 30-34, 1979

Evidence for a new, oxygen-regulated biosynthetic pathway for the pyrimidine moiety of thiamine in Salmonella typhimurium. Journal of Bacteriology 174(5): 1515-1521, 1992

Inhibition of intimal proliferation after vein grafting by chitosan nanoparticle with proliferation cell nuclear antigen-antisense oligo deoxy nucleotides. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 21(12): 1348-1354, 2007

Nmr studies of hydrogen bonded complexes of oligo nucleotides in aqueous solution part 1 deoxy di nucleotides deoxy guanylate deoxy cytidylate and deoxy guanylate deoxy thymidylate. Biochemical and Biophysical Research Communications 62(4): 1025-1032, 1975

Enzymes involved in biosynthesis of pyrimidine nucleotides and cell proliferation. Sebastien, P (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale) 203 Mecanismes Des Pneumopathies Professionnelles; (National Institute Of Health And Medical Research Colloquium 203 Mechanisms in Occupational Lung Diseases); 2nd Conference Of The Gerp (Groupe D'etude Et De Recherches Sur Les Pneumopathies Professionnelles; Study Group on Occupational Lung Disease) 14-15, 1989 Xiii+416p Les Editions Inserm: Paris, 1991

Involvement of purine biosynthetic genes in aerobic function of the alternative pyrimidine biosynthetic pathway in Salmonella typhimurium. Abstracts of the General Meeting of the American Society for Microbiology 96: 562, 1996

Differential control of cell cycle, proliferation, and survival of primary T lymphocytes by purine and pyrimidine nucleotides. Journal of Immunology 170(10): 4986-4995, 2003

Poly nucleotides 63. solution conformation of oligo deoxy ribo nucleotides containing an alternating deoxy cytidylate deoxy guanylate sequence which can form a left handed double helix. Chemical and Pharmaceutical Bulletin 29(12): 3573-3585, 1981

A cytokinetic approach to determine the range of O2-dependence of pyrimidine(deoxy)nucleotide biosynthesis relevant for cell proliferation. Cell Proliferation 25(3): 169-179, 1992

Preparative isolation of hexa pyrimidine hepta pyrimidine octa pyrimidine nona pyrimidine and deca pyrimidine oligo nucleotides from hydrolysates of depurinated herring sperm dna. Journal of Chromatography 284(2): 409-432, 1984

Inhibition of de novo pyrimidine synthesis in growing potato tubers leads to a compensatory stimulation of the pyrimidine salvage pathway and a subsequent increase in biosynthetic performance. Plant Cell 17(7): 2077-2088, 2005

Inhibition of de Novo Pyrimidine Synthesis in Growing Potato Tubers Leads to a Compensatory Stimulation of the Pyrimidine Salvage Pathway and a Subsequent Increase in Biosynthetic Performance. The Plant Cell 17(7): 2077-2088, 2005

The metabolism of pyrimidine deoxy-nucleotides during Rana pipiens development. Diss: 2729, 1969