EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

The cDNA sequence coding for prepro-PGS (prepro-magainins) and aspects of the processing of this prepro-polypeptide


The cDNA sequence coding for prepro-PGS (prepro-magainins) and aspects of the processing of this prepro-polypeptide



Journal of Biological Chemistry 263(12): 5745-5751



ISSN/ISBN: 0021-9258

PMID: 2833514

Amphibian skin is well known as a source of peptides homologous to bioactive peptides found in mammalian gut and brain. A systematic investigation of the skin secretions from Xenopus laevis revealed several peptides not derivable from known precursors. The sequence elucidation, utilizing fast atom bombardment/mass spectrometry, of two peptides, PGS and PGS Gly-10;Lys-22, is reported. These have been independently characterized and named magainins and found to have antimicrobial activity. A mixed sequence oligonucleotide probe complementary to the mRNA sequence coding for PGS was synthesized and used to screen a Xenopus skin cDNA library. A full length cDNA species encoding prepro-PGS was isolated and characterized, and its sequence is reported. The deduced precursor sequence was found to contain one copy of PGS Gly-10;Lys-22 and five copies of PGS. The proteolytic processing of this prepro-polypeptide is discussed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 018161969

Download citation: RISBibTeXText

Related references

Ether anesthesia differentially affects the content of prepro-corticotropin-releasing hormone, prepro-neurotensin/neuromedin N and prepro-enkephalin mRNAs in the hypothalamic paraventricular nucleus of the rat. Brain Research 544(2): 353-357, 1991

Prepro-TRH 178-199 secretion is inhibited by dexamethasone in AtT-20 cells transfected with the rat prepro-TRH cDNA. Society for Neuroscience Abstracts 23(1-2): 2053, 1997

Processing of thyrotropin-releasing hormone prohormone (pro-TRH) generates pro-TRH-connecting peptides. Identification and characterization of prepro-TRH-(160-169) and prepro-TRH-(178-199) in the rat nervous system. Journal of Biological Chemistry 263(32): 17189-17196, 1988

Nucleotide sequence of a cDNA coding for the amino-terminal region of human prepro α1(III) collagen. Nucleic Acids Research 16(14): 7201-7201, 1988

Prepro-hypocretin (prepro-orexin) expression is unaffected by short-term sleep deprivation in rats and mice. Sleep 23(7): 867-874, 2000

Nucleotide sequence of a cDNA coding for the amino-terminal region of human prepro alpha 1(III) collagen. Nucleic Acids Research 16(14b): 7201, 1988

Low cerebrospinal fluid concentrations of peptide histidine valine and somatostatin-28 in Alzheimer's disease: altered processing of prepro-vasoactive intestinal peptide and prepro-somatostatin. Neuropeptides 29(6): 325-330, 1995

A splicing variant for the prepro-mammalian gonadotropin-releasing hormone (prepro-mGnRH) mRNA is present in the brain and various peripheral tissues of the Japanese eel. Zoological Science 16(4): 645-651, 1999

A prepro-TRH connecting peptide (prepro-TRH 160-169) potentiates TRH-induced TSH release from rat perifused pituitaries by stimulating dihydropyridine- and omega-conotoxin-sensitive Ca2+ channels. Neuroendocrinology 54(6): 559-565, 1991

Cell-type specific interaction of endothelin and the nitric oxide system: pattern of prepro-ET-1 expression in kidneys of L-NAME treated prepro-ET-1 promoter-lacZ-transgenic mice. Journal of Physiology 581(Pt 3): 1173-1181, 2007

Nucleotide sequence of a porcine prepro atrial natriuretic peptide (ANP) cDNA. Nucleic Acids Research 18(22): 6704, 1990

Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence. Nucleic Acids Research 12(11): 4481-4492, 1984

Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence. Journal of Biotechnology 167(2): 94-100, 2013

Nucleotide sequence of a complementary dna coding for the amino terminal region of human prepro alpha iii collagen. Nucleic Acids Research 16(14 Part B): 7201, 1988

Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor. Journal of Biological Chemistry 269(4): 2440-2446, 1994