EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

The complete amino acid sequence of monkey progastricsin


The complete amino acid sequence of monkey progastricsin



Journal of Biological Chemistry 261(10): 4406-4419



ISSN/ISBN: 0021-9258

PMID: 3514597

The complete amino acid sequence of progastricsin from the Japanese monkey (Macaca fuscata) was determined. Progastricsin is composed of 374 residues, including the gastricsin moiety of 331 residues and the activation segment of 43 residues. Upon activation under acidic conditions, progastricsin was converted to gastricsin via the intermediate protein species. NH2-terminal sequence determination of these protein species enabled us to deduce the NH2-terminal 78-residue sequence of progastricsin, including the 43-residue activation segment. The complete sequence of the gastricsin moiety was determined using peptide fragments obtained by several chemical and enzymatic cleavages. The molecular weight of progastricsin was determined to be 40,785. As compared with pepsinogen A of the same monkey species, deletion of 4 residues and insertion of 5 residues were observed. Although monkey progastricsin and pepsinogen A have highly homologous sequences around the two active site aspartyl residues, the homology between these proteins is rather small (49% identity). This indicates that progastricsin diverged from pepsinogen A in the early phase of the evolution of gastric aspartyl proteinases.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 018163035

Download citation: RISBibTeXText

Related references

Separation of porcine pepsinogen A and progastricsin. Sequencing of the first 73 amino acid residues in progastricsin. Biochimica et Biophysica Acta 1121(1-2): 75-82, 1992

The complete amino acid sequence of monkey pepsinogen A. Journal of Biological Chemistry 261(10): 4395-4405, 1986

Human progastricsin. Analysis of intermediates during activation into gastricsin and determination of the amino acid sequence of the propart. European Journal of Biochemistry 128(1): 63-70, 1982

Monkey Pepsinogens and Pepsins. Vii. Analysis of the Activation Process and Determination of the Nh2-Terminal 60-Residue Sequence of Japanese Monkey Progastricsin, and Molecular Evolution of Pepsinogens1. The Journal of Biochemistry 97(4): 1235-1246, 1985

Complete amino acid sequence of human phosphoglycerate kinase. Cyanogen bromide peptides and complete amino acid sequence. Journal of Biological Chemistry 255(13): 6412-6420, 1980

Amino acid sequence of lima bean d protease inhibitor component iv part 2 isolation and sequence determination of the chymotryptic peptides and the complete amino acid sequence. European Journal Of Biochemistry: 515-523, 1971

Amino acid sequence of lima bean protease inhibitor component IV. 2. Isolation and sequence determination of the chymotryptic peptides and the complete amino acid sequence. European Journal of Biochemistry 18(4): 515-523, 1971

Monkey pepsinogens and pepsins. 7. Analysis of the activation process and determination of the NH2-terminal 60-residue sequence of Japanese monkey progastricsin, and molecular evolution of pepsinogens. Journal of Biochemistry (Tokyo): 974: 1235-1246, 1985

Monkey pepsinogens and pepsins. VII. Analysis of the activation process and determination of the NH2-terminal 60-residue sequence of Japanese monkey progastricsin, and molecular evolution of pepsinogens. Journal of Biochemistry 97(4): 1235-1246, 1985

Complete amino acid sequences of human phospho glycerate kinase ec 2.7.2.3 cyanogen bromide peptides and complete amino acid sequence. Journal of Biological Chemistry 255(13): 6412-6414, 1980

The amino acid sequence of the A-protein a-subunit of the tryptophan synthetase of Escherichia coli: V. Order of tryptic peptides and the complete amino acid sequence. J Biol Chem 242(22): 5442-5446, 1967

The amino acid sequence of a lambda type Bence-Jones protein. 3. The complete amino acid sequence and the location of the disulfide bridges. Journal of Biological Chemistry 245(8): 2171-2176, 1970

The complete amino acid sequence of growth hormone and partial amino acid sequence of prolactin and somatolactin from sea perch (Lateolabrax japonicus). Aquaculture 201(1/2): 117-136, 2001

Complete amino acid sequence of human phosphoglycerate kinase. Isolation and amino acid sequence of tryptic peptides. Journal of Biological Chemistry 255(13): 6408-6411, 1980

Complete amino acid sequence of mitochondrial aspartate aminotransferase from pig heart muscle. Peptide ordering procedures and the complete sequence. Journal of Biological Chemistry 255(13): 6153-6159, 1980