+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go

The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go

Journal of Biological Chemistry 262(2): 757-761

ISSN/ISBN: 0021-9258

PMID: 3027067

The structures of the guanosine 5'O-(3-thio)triphosphate (GTP gamma S)-containing guanine nucleotide-binding regulatory proteins (G proteins) are distinct from those of the GDP-containing forms. One indication of the conformational change caused by GTP gamma S is a Mg2+-sensitive increase in the intensity of the proteins' tryptophan fluorescence (Higashijima, T., Ferguson, K.M., Sternweis, P.C., Ross, E.M., Smigel, M.D., Gilman, A.G. (1987), J. Biol. Chem., 262, 762-766). GTP causes a similar change in the fluorescence of Go, a G protein from bovine brain. When Mg2+ is also present, the increase in fluorescence is transient, and the rate of decline in the intensity of the fluorescence is the same as the rate of GTP hydrolysis by the protein. The steady-state rate of hydrolysis of GTP by Go (0.3-0.4/min) is slower than the catalytic rate of the protein (2/min), because the rate-limiting step in the reaction is the release of GDP.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 018165289

Download citation: RISBibTeXText

Related references

Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues. Journal of Biological Chemistry 280(9): 7712-7719, 2005

Ha-ras proteins exhibit GTPase activity: point mutations that activate Ha-ras gene products result in decreased GTPase activity. Proceedings of the National Academy of Sciences of the United States of America 82(2): 376-380, 1985

Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of Virology 90(9): 4412-4426, 2016

The pharmacological activity of some new fluorescent small molecule histamine H2 receptor (H2-R) ligands, derived from aminopotentidine and squaramide, in the GTPase assay. Revista Medico-Chirurgicala a Societatii de Medici Si Naturalisti Din Iasi 114(1): 255-259, 2010

Fluorescent molecular probes I. The synthesis and biological properties of an ELF beta-glucuronidase substrate that yields fluorescent precipitates at the enzymatic activity sites. Tetrahedron 53(21): 7159-7164, 1997

Era GTPase of Escherichia coli: binding to 16S rRNA and modulation of GTPase activity by RNA and carbohydrates. Microbiology 146: 1071-1083, 2000

Rho GTPase-activating proteins: Regulators of Rho GTPase activity in neuronal development and CNS diseases. Molecular and Cellular Neurosciences 80: 18-31, 2017

Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima. Journal of Bacteriology 182(24): 7078-7082, 2000

Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity. Journal of Biological Chemistry 270(38): 22473-22477, 1995

Characterization of Rad, a new member of Ras/GTPase superfamily, and its regulation by a unique GTPase-activating protein (GAP)-like activity. Journal of Biological Chemistry 270(9): 4805-4812, 1995

Inhibition of the ras p21 GTPase-activating protein-stimulated GTPase activity of c-Ha-ras p21 by smg p21 having the same putative effector domain as ras p21s. Journal of Biological Chemistry 265(13): 7104-7107, 1990

Regulation of the GTPase activity of Rho-family GTPase by homodimer formation. FASEB Journal 12(8): A1370, 1998

Role of nucleotide binding and GTPase domain dimerization in dynamin-like myxovirus resistance protein A for GTPase activation and antiviral activity. Journal of Biological Chemistry 290(20): 12779-12792, 2015

Binding of the H-ras p21 GTPase activating protein by the activated epidermal growth factor receptor leads to inhibition of the p21 GTPase activity in vitro. Biochemistry 31(28): 6361-6365, 1992

Conformational activation of the EF-Tu . GTPase activity. Stimulation of the 2'(3')-O-L-phenylalanyladenosine-promoted EF-Tu . GTPase upon binding of the tRNAPhe lacking the terminal adenosine residue. Biochimica et Biophysica Acta 699(3): 293-296, 1982