+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

The effect of tRNA binding on the structure of 5 S RNA in Escherichia coli. A chemical modification study

The effect of tRNA binding on the structure of 5 S RNA in Escherichia coli. A chemical modification study

Journal of Biological Chemistry 259(1): 491-496

ISSN/ISBN: 0021-9258

PMID: 6200475

The structure of 5 S RNA within the 70 S ribosome from Escherichia coli was studied using the chemical reagent kethoxal (alpha-keto-beta-ethoxybutyraldehyde) to modify accessible guanosines. The modification pattern of 5 S RNA from free 70 S ribosomes was compared with that of poly(U) programmed ribosomes where tRNA had been bound to both the A- and P-sites. Binding to the ribosomal A-site was achieved enzymatically using the elongation factor Tu and GTP in the presence of deacylated tRNA which blocks the ribosomal P-site. Modified guanosines were identified after partial RNase T1 hydrolysis and separation of the hydrolysis products on sequencing gels. Binding of tRNA to the ribosome leads to a strong protection of 5 S RNA guanosine G-41 and to some degree G-44 from kethoxal modification. The limited RNase T1 hydrolysis pattern provides evidence for the existence of a 5 S RNA conformation different from the known 5 S RNA A- and B-forms which are characterized by their gel electrophoretic mobility. The importance of 5 S RNA for the binding of tRNA to the ribosome is discussed.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 018166017

Download citation: RISBibTeXText

Related references

Effect of transfer rna binding on the structure of 5s rna in escherichia coli a chemical modification study. Journal of Biological Chemistry 259(1): 491, 1984

Chemical modification of the complex of tRNA Phe with phenylalanyl-tRNA synthetase from Escherichia coli. Molekuliarnaia Biologiia 13(5): 994-1000, 1979

Catalytic mechanism of isoleucyl-tRNA synthetase of Escherichia coli K10. Effect of pH and chemical modification. Biochemistry 19(23): 5403-5411, 1980

Chemical modification of phenylalanyl-tRNA synthetase and ribosomes of Escherichia coli with derivatives of tRNA-Phe carrying photoreactive groups on guanosine residues. Molekuliarnaia Biologiia 14(3): 531-538, 1980

Chemical modification in situ of Escherichia coli 30 S ribosomal proteins by the site-specific reagent pyridoxal phosphate. Inactivation of the aminoacyl-tRNA and mRNA binding sites. Journal of Biological Chemistry 258(1): 150-156, 1983

Comparative analysis of interaction sites of Thermus thermophilus and Escherichia coli tRNA(Tyr) with homologous aminoacyl-tRNA synthetases by means of chemical modification and nuclease hydrolysis. Bioorganicheskaia Khimiia 24(8): 593-600, 1998

Effect of Modification of tRNA Nucleotide 37 on the tRNA Interaction with the A and P Sites of the Escherichia coli 70S Ribosome. Molecular Biology 40(4): 7-610, 2006

The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli. Molekuliarnaia Biologiia 40(4): 669-683, 2006

Solution structure of the anticodon-binding domain of Escherichia coli lysyl-tRNA synthetase and studies of its interaction with tRNA(Lys). Journal of Molecular Biology 253(1): 100-113, 1995

tRNA recognition for modification: solution probing of tRNA complexed with Escherichia coli tRNA (guanosine-1) methyltransferase. Rna 3(11): 1327-1336, 1997

Affinity modification of Escherichia coli ribosomes near the acceptor tRNA-binding site. Molekuliarnaia Biologiia 18(5): 1287-1296, 1984

Study of the photoaffinity modification of Escherichia coli ribosomes near the donor tRNA-binding center. Molekuliarnaia Biologiia 19(2): 545-552, 1985

Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli. Protein Science: a Publication of the Protein Society 20(7): 1105-1113, 2011

Conversion of Escherichia coli tRNATrp to glutamine-accepting tRNA by chemical modification with sodium bisulfite. Febs Letters 51(1): 325-329, 1975

Study of the poly(U)-dependent interaction of tRNA(Phe) with the P-site of Escherichia coli ribosomes by chemical modification with nitrosoethylurea. Molekuliarnaia Biologiia 28(4): 926-931, 1994