EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

The periodic synthesis of tubulin in the Physarum cell cycle. Characterization of Physarum tubulins by affinity for monoclonal antibodies and by peptide mapping


The periodic synthesis of tubulin in the Physarum cell cycle. Characterization of Physarum tubulins by affinity for monoclonal antibodies and by peptide mapping



Journal of Biological Chemistry 258(2): 1352-1356



ISSN/ISBN: 0021-9258

PMID: 6822502

Polypeptides preferentially labeled in the G2 phase of the synchronous nuclear replication cycle of Physarum macroplasmodia were compared in electrophoretic mobility and peptide map with the tubulins enriched from Physarum myxamoebae. One major and one minor fluorographic species match the myxamoebal alpha and beta chains, respectively. Thus, tubulins are among the proteins of Physarum selectively synthesized before nuclear division. A third species P, prominently labeled in premitotic plasmodia, is distinct from the two myxamoebal tubulins even though it co-polymerizes with microtubules. The nature of P remains unknown. Two rat monoclonal antibodies directed against yeast tubulin were found to bind selectively to the alpha tubulin of porcine brain. These served to confirm the assignment of the 50,000-dalton Physarum myxamoebal tubulin as an alpha-like polypeptide.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 018175366

Download citation: RISBibTeXText

Related references

Ther periodic synthesis of tubulin in the Physarum cell cycle. Journal of Biological Chemistry 258(2): 1352-1356, 1983

Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum. Journal of Cell Biology 99(1 Pt 1): 155-165, 1984

Tubulin synthesis tubulin rna level and assembly of micro tubules during the synchronous cell cycle of physarum plasmodia. Journal of Cell Biology 97(5 Part 2): 218A, 1983

Part 1 || Cell Cycle Regulation of Tubulin RNA Level, Tubulin Protein Synthesis, and Assembly of Microtubules in Physarum. Journal of Cell Biology 99(1): 155-165, 1984

Regulation of tubulin synthesis in the physarum cell cycle. Texas Journal of Science 35(4): 335-336, 1984

A comparison of tubulins from mammalian brain and Physarum polycephalum using SDS-polyacrylamide gel electrophorsis and peptide mapping. Febs Letters 115(2): 301-305, 1980

Periodic synthesis of microtubular proteins in the cell cycle of Physarum. Proceedings of the National Academy of Sciences of the United States of America 78(8): 5000-5004, 1981

Regulation of tubulin synthesis during the cell cycle in the synchronous plasmodia of Physarum polycephalum. Journal of Cellular Physiology 145(1): 120-128, 1990

Negative control of periodic tubulin gene expression in the mitotic cycle of Physarum polycephalum. European Journal of Cell Biology 43(2): 179-181, 1987

Evolution and patterns of expression of the physarum multi tubulin family analyzed by the use of monoclonal antibodies. Timberlake, W E (Ed ) Ucla (University Of California-Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 34 Molecular Genetics Of Filamentous Fungi; Meeting, Keystone, Colo , Usa, Apr 13-19, 1985 Xix+465p Alan R Liss, Inc : New York, N Y , Usa Illus 265-276, 1985, 1986

Evolution and patterns of expression of the Physarum multi-tubulin family analysed by the use of monoclonal antibodies. UCLA symposia on molecular and cellular biology 4(34): 265-275, 1985

Synthesis and characterization of nuclear matrix proteins during the cell cycle of Physarum polycephalum. Cell Biology International Reports 16(11): 1169-1175, 1992

Tubulin half life during the cell cycle of physarum plasmodia. Biological Chemistry Hoppe-Seyler 369(10): 1076-1077, 1988

Periodic and continuous enzyme synthesis in the synchronous mitotic cycle of physarum polycephalum. Hoppe-Seyler's Zeitschrift fuer Physiologische Chemie 357(3): 259, 1976

Regulation of tubulin gene expression through the life and cell cycle of Physarum polycephalum. Dissertation Abstracts International B Sciences and Engineering, 488: 2215, 1988