+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Trans-acting transposase mutant from Tn5



Trans-acting transposase mutant from Tn5



Proceedings of the National Academy of Sciences of the United States of America 88(14): 6072-6076



Transposition of Tn5 and of its component insertion sequence IS50R is regulated through the action of two proteins it encodes: a cis-acting transposase, Tnp, and a trans-acting inhibitor of transposition, Inh. The mechanism of the cis-acting Tnp and the relevance of inhibition to cis action have been addressed in the current study. A specific colony morphology assay for transposition of Tn5 was shown to be sensitive to Inh produced in trans and was used to screen for mutants in Inh and/or Tnp with altered regulation. A dominant mutant in IS50R that promotes transposition in trans was isolated and characterized. The mutant (449F) carries a Leu----Phe mutation at position 449 in Tnp. This mutation reduces the frequency of Tn5 or IS50R transposition in cis but allows Tnp-449F to act as efficiently in trans as it does in cis. Tnp-449F is sensitive to inhibition and, furthermore, Inh-449F is a competent inhibitor in trans. These results show that Tnp-449F is a trans-acting transposase, unlike wild-type Tnp, which is cis-acting.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018188812

Download citation: RISBibTeXText

PMID: 1648727

DOI: 10.2307/2357440


Related references

Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase. Journal of Molecular Biology 276(5): 913-925, 1998

Fruitful EMS mutagenesis: search for suppressors of lg1 and discovery of an adh1 trans-acting mutant. Maize Genetics Cooperation Newsletter ( 69): 22, 1995

Is a trans-acting defect in RNA splicing responsible for the Arabidopsis gametophytic factor 1 mutant phenotype?. Plant Biology (Rockville) : 43, 2000

An isotype-specific trans-acting factor is defective in a mutant B cell line that expresses HLA-DQ, but not -DR or -DP. Journal of Experimental Medicine 173(3): 629-637, 1991

Characterization of a soda mutant of escherichia coli cooperativity between cis and trans acting regulatory elements. Abstracts of the General Meeting of the American Society for Microbiology 92: 221, 1992

Cis-acting elements for in trans complementation of replication-defective mutant of tobacco mosaic virus. Virology 191(1): 454-458, 1992

An isotype specific trans acting factor is defective in a mutant b cell line that expresses hla dq but not hla dr or hla dp. Journal of Experimental Medicine 173(3): 629-638, 1991

Trans-acting suppressors that affect the haplo-X chromatin packaging of the giant (gt) mutant of Drosophila melanogaster. Proceedings of the Zoological Society 47(2): 107-123, 1995

A trans-acting factor for activation of transcription is defective in the xrs-5 mutant of the Chinese hamster ovary cell line. Mutation Research. 294(2): 101-108, 1993

Sequence of mal64 mutant alleles encoding trans acting factors for constitutive expression of the maltose fermentative genes. Journal of Cellular Biochemistry Supplement (13 PART E): 52, 1989

A Thy-1- mutant defining a gene acting in trans position to regulate cell-surface Thy-1 glycoprotein expression and Thy-1 messenger RNA content. Immunogenetics 23(5): 312-321, 1986

A multifunctional expression vector for an anti-HIV-1 ribozyme that produces a 5'- and 3'-trimmed trans-acting ribozyme, targeted against HIV-1 RNA, and cis-acting ribozymes that are designed to bind to and thereby sequester trans-activator proteins such as Tat and Rev. Nucleic Acids Research 22(23): 5060-5067, 1994

Genetic variation in apo a iv messenger rna levels is acting controlled post transcriptionally by a combination of cis acting and trans acting regulatory elements. Circulation 82(4 SUPPL 3): III4, 1990

Binding of trans-dominant mutant Rev protein of human immunodeficiency virus type 1 to the cis-acting Rev-responsive element does not affect the fate of viral mRNA. New Biologist 2(12): 1111-1122, 1990

Class ii mhc specific trans acting transcription factors defined by class ii mhc negative mutant b cell lines. Federation Proceedings 46(4): 1196, 1987