EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Transcriptional activation of heat-shock genes in eukaryotes


Transcriptional activation of heat-shock genes in eukaryotes



Biochemistry and Cell Biology 66(6): 584-593



ISSN/ISBN: 0829-8211

PMID: 3048332

DOI: 10.1139/o88-069

Prokaryotes and eukaryotes respond to thermal or various chemical stresses by the rapid induction of a group of genes collectively referred to as the heat shock genes. In eucaryotes, the expression of these genes is primarily regulated at the transcriptional level. The early observations that transfected heat shock genes were inducible in heterologous systems suggested the existence of common regulatory elements in these ubiquitous genes. Sequence analysis of cloned Drosophila heat shock genes revealed a conserved 14 base pair (bp) inverted repeat, which is essential for heat induction. This regulatory sequence, referred to as the heat shock element (HSE), is found in multiple imperfect copies upstream of the TATA box of all heat shock genes. While studies in heterologous systems indicated that a single copy of HSE was sufficient for inducibility, further analysis in homologous assays suggests that multiple HSE can act in a cooperative way and that the efficiency of transcriptional activation is related, within limits, to the number of HSE. Comparative analysis of heat shock genes reveals that HSE can be positioned at different distances from the TATA box in either orientation, a behavior reminiscent of enhancer elements. However, the presence of HSE does not necessarily confer heat inducibility, as shown by their presence in the constitutively expressed but non-heat-inducible homologous cognate genes. Footprinting and nuclease mapping have been used to show that a protein factor (HSTF: heat shock transcription factor) binds to the HSE element, activating heat shock gene transcription in a dose-dependent manner. The recent progress in the isolation and characterization of HSTF in Drosophila, yeast, and human cells is reviewed. Finally, different models suggested to account for the positive regulation of heat shock genes by the HSTF are presented.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018189548

Download citation: RISBibTeXText

Related references

Activation of heat-shock genes in eukaryotes. Trends in Genetics 1: 31-35, 1985

Binding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila. Nucleic Acids Research 23(23): 4799-4804, 1995

Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5°C. Insect Biochemistry and Molecular Biology 41(11): 843-851, 2011

Molecular events involved in transcriptional activation of heat shock genes become progressively refractory to heat stimulation during aging of human diploid fibroblasts. Journal of Cellular Physiology 149(3): 560-566, 1991

Cells in stress: transcriptional activation of heat shock genes. Science 259(5100): 1409-1410, 1993

Transcriptional regulation and binding of heat shock factor 1 and heat shock factor 2 to 32 human heat shock genes during thermal stress and differentiation. Cell Stress and Chaperones 9(1): 21-28, 2004

yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae. Current Genetics 29(2): 103-105, 1996

Transcriptional activation of mouse cytosolic chaperonin CCT subunit genes by heat shock factors HSF1 and HSF2. FEBS Letters 461(1-2): 125-129, 1999

Fever transforms heat shock factor-1 from a heat shock-induced transcriptional activator to a repressor of proinflammatory cytokine genes. Pediatric Research 49(4 Part 2): 246A, 2001

The transcriptional regulation of heat shock genes: a plethora of heat shock factors and regulatory conditions. Exs 77: 139-163, 1996

Transcriptional activation of a heat shock gene promoter in sunflower embryos: synergism between Abi3 and heat shock factors. The Plant Journal 20(5): 601-610, 1999

DNA binding of heat shock factor to the heat shock element is insufficient for transcriptional activation in murine erythroleukemia cells. Molecular and Cellular Biology 10(4): 1600-1608, 1990

Transcriptional activation of a heat shock gene promoter in sunflower embryos: synergism between ABI3 and heat shock factors. Plant Journal: for Cell and Molecular Biology 20(5): 601-610, 1999

Different mechanisms are involved in the transcriptional activation by yeast heat shock transcription factor through two different types of heat shock elements. Journal of Biological Chemistry 282(14): 10333-10340, 2007

Gene expression of 70 kDa heat shock protein of Candida albicans: transcriptional activation and response to heat shock. Medical Mycology 40(5): 471-478, 2002