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Trehalose-containing lipooligosaccharides. A new class of species-specific antigens from Mycobacterium


, : Trehalose-containing lipooligosaccharides. A new class of species-specific antigens from Mycobacterium. Journal of Biological Chemistry 258(17): 10481-7

Mycobacterium kansasii is characterized by the presence of seven species-specific neutral lipooligosaccharide antigens. All react with hyperimmune anti-M. kansasii serum in enzyme-linked immunosorbent assays, and the more glycosylated members also react by gel diffusion. Both the native glycolipids and their inherent oligosaccharides were purified and the major features of their unique structures determined by acetolysis, partial acid cleavage, 1H-NMR and 13C-NMR, and chemical ionization and electron impact mass spectrometry of the permethylated products. They have in common a tetraglucose "core," beta-D-Glcp-(1 leads to 3)-beta-D-Glcp-(1 leads to 4)- alpha-D-Glcp(1 leads to 1)-alpha-D-Glcp where Glcp is glucopyranose, distinguished by the presence of an alpha,alpha-trehalose substituent. Variable residues of xylose, 3-O-methylrhamnose, fucose, and a novel N-acyl aminosugar, (4,6-dideoxy-2-O-methyl-3-C-methyl-4-(2'-methoxypropionamido)hexose; proof of this structure is not given) are linked to the core and the resulting oligosaccharides are acylated with 2,4-dimethyltetradecanoyl and acetyl functions to present a familial arrangement. Related but species-specific lipooligosaccharides typify Mycobacterium szulgai and apparently a host of other atypical mycobacteria. Thus, the antigenicity, and perhaps other features such as pathogenesis and drug resistance, of most atypical mycobacteria may be rationalized in terms of these new found cell wall lipooligosaccharides and the previously described glycopeptidolipids.

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Accession: 018192760

PMID: 6411715

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Related references

W.H.nter S.; Murphy R.C.; Clay K.; Goren M.B.; Brennan P.J., 1983: Trehalose containing lipo oligo saccharides a new class of species specific antigens from mycobacterium kansasii. M. kansasii is characterized by the presence of 7 species-specific neutral lipooligosaccharide antigens. All react with hyperimmune anti-M. kansasii serum in enzyme-linked immunosorbent assays and the more glycosylated members also react by gel di...

Muñoz, M.; Raynaud, C.; Lanéelle, M.A.; Julián, E.; López Marín, L.M.; Silve, G.; Ausina, V.; Daffé, M.; Luquin, M., 1998: Seroreactive species-specific lipooligosaccharides of Mycobacterium mucogenicum sp. nov. (formerly Mycobacterium chelonae-like organisms): identification and chemical characterization. Strains of the new species Mycobacterium mucogenicum exhibit physiological and biochemical features very similar to those of the other species of the Mycobacterium fortuitum complex. To define taxonomic criteria for easy identification of M. mucog...

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Besra, G., S.; Mcneil, M., R.; Khoo, K.-Hooi; Dell, A.; Morris, H., R.; Brennan, P., J., 1993: Trehalose-containing lipooligosaccharides of Mycobacterium gordonae: Presence of a mono-O-methyltetra-O-acyltrehalose core and branching in the oligosaccharide backbone. Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labi...

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Daffe, M.; McNeil, M.; Brennan, P.J., 1991: Novel type-specific lipooligosaccharides from Mycobacterium tuberculosis. Mycobacterium tuberculosis (strain Canetti) is characterized by the presence of two novel glycolipids of the alkali-labile, trehalose-containing lipooligosaccharide class. Their structures were established by permethylation, partial acid hydrolysi...

Tórtola, M.T.; Lanéelle, M.A.; Martín-Casabona, N., 1996: Comparison of two 2,3-diacyl trehalose antigens from Mycobacterium tuberculosis and Mycobacterium fortuitum for serology in tuberculosis patients. Immunoglobulin G antibodies against two 2,3-diacyl trehalose (DAT) antigens from Mycobacterium tuberculosis (DAT-T) and Mycobacterium fortuitum (DAT-F) were studied by enzyme-linked immunosorbent assay of 356 serum samples. The sera were obtained...

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