EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Unique organization of Leptospira interrogans rRNA genes


Unique organization of Leptospira interrogans rRNA genes



Journal of Bacteriology 171(11): 5763-5767



ISSN/ISBN: 0021-9193

PMID: 2808297

We cloned Sau3AI fragments containing the rRNA genes for Leptospira interrogans serovar canicola strain Moulton in the BamHI site of lambda EMBL3 bacteriophage DNA. Physical maps of the fragments were constructed, and the locations of the rRNA genes were determined by Southern blot hybridization and S1 protection. Each fragment of the 23S or the 16S rRNA gene contained at least one copy of the 23S or the 16S sequence. Genomic hybridization showed that there were two genes for the 23S rRNA and the 16S rRNA but only one gene for the 5S rRNA on the chromosome of L. interrogans. The results revealed the important fact that each rRNA gene is located far from the other rRNA genes. Our findings, accordingly, also suggest that these rRNA genes are expressed independently in this organism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 018198825

Download citation: RISBibTeXText

Related references

Unique ribosome structure of Leptospira interrogans is composed of four rRNA components. Journal of Bacteriology 172(6): 3478-3480, 1990

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae. Journal of Bacteriology 174(23): 7566-7571, 1992

The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa. Microbiology and Immunology 33(6): 459-466, 1989

Comparison of flanking regions of the 5S ribosomal ribonucleic acid genes in Leptospira biflexa and Leptospira interrogans. Chemical and Pharmaceutical Bulletin 40(2): 544-546, 1992

Isolation and characterization of the 5S rRNA gene of Leptospira interrogans. Journal of Bacteriology 172(6): 3264-3268, 1990

Repetitive sequence of Leptospira interrogans serovar icterohaemorrhagiae strain Ictero No. 1: a sensitive probe for demonstration of Leptospira interrogans strains. Microbiology and Immunology 43(7): 669-678, 1999

Isolation of antigenic variants of leptospiras from puppies and pigs experimentally infected with leptospira interrogans canicola and leptospira interrogans pomona. Japanese Journal of Veterinary Science 42(2): 177-186, 1980

Use of hamster anti sera in the preliminary differentiation of leptospira interrogans hardjo and leptospira interrogans balcanica. Journal of Medical Microbiology 11(3): 351-354, 1978

Hemolysis as a means of distinguishing between leptospira interrogans balcanica and leptospira interrogans hardjo. Journal of Medical Microbiology 13(3): 477-482, 1980

Studies on the seroprevalence of antibodies against Leptospira interrogans in hunters and wild boar from south-eastern Austria Untersuchung von Jaegern und Schwarzwild auf die Seropraevalenz von Antikoerpern gegenueber Leptospira interrogans in Suedostoesterreich. Zeitschrift fuer Jagdwissenschaft. Maerz; 481: 60-65, 2002

Development of a Multiple-Locus Variable Number of Tandem Repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia. Annals of Clinical Microbiology and Antimicrobials 4: 10, 2005

Experimental infections of brush tailed possums trichosurus vulpecula common wombats vombatus ursinus and water rats hydromys chrysogaster with serovars leptospira interrogans balcanica and leptospira interrogans hardjo. Australian Journal of Experimental Biology and Medical Science 57(3): 231-240, 1979

Nucleotide sequence of a 16S rRNA gene for Leptospira interrogans serovar canicola strain Moulton. Nucleic Acids Research 18(2): 366, 1990

First report on Leptospira interrogans in the sigmodontine rodent Scapteromys aquaticus Primeiro relato da presença de Leptospira interrogans em roedores sigmodontíneos Scapteromys aquaticus. Revista Panamericana de Salud Publica 42: E83, 2018

Analysis of field strains of Leptospira interrogans serovar hebdomadis and australis by MRSP of rRNA gene. Zhongguo Meijieshengwuxue ji Kongzhi Zazhi = Chinese Journal of Vector Biology and Control 11(3): 201-203, 2000