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Utilization of side-chain precursors for penicillin biosynthesis in a high-producing strain of Penicillium chrysogenum


, : Utilization of side-chain precursors for penicillin biosynthesis in a high-producing strain of Penicillium chrysogenum. Applied Microbiology and Biotechnology 40(6): 883-887

Utilization of the side-chain precursors phenoxyacetic acid (POA) and phenylacetic acid (PA) for penicillin biosynthesis by Penicillium chrysogenum was studied in shake flasks. Precursor uptake and penicillin production were followed by HPLC analysis of precursors and products in the medium and in the cells. P. chrysogenum used both POA and PA as precursors, producing phenoxymethylpenicillin (penicillin V) and benzylpenicillin (penicillin G), respectively. If both precursors were present simultaneously, the formation of penicillin V was blocked and only penicillin G was produced. When PA was added at different times to cells that were induced initially for POA utilization and were producing penicillin V, the POA utilization and penicillin V formation were blocked, whereas the cells started utilizing PA and produced penicillin G. The blocking of the POA turnover lasted for as long as PA was present in the medium. If POA was added to cultures induced initially for PA utilization and producing penicillin G, this continued irrespective of the presence of POA. Utilization of POA increased concomitant with depletion of PA from the medium. Analysis of cellular pools from a growing cell system with POA as precursor to which PA was added after 48 h showed that the cellular concentration of POA was kept high without production of penicillin V and at a concentration comparable to the concentration in the medium. The cellular concentration of POA was higher than the concentration of PA that was utilized for penicillin G production.

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Accession: 018201748

PMID: 7764573

DOI: 10.1007/BF00173993

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Related references

Eriksen, S.H.vn; Jensen, B.; Schneider, I.; Kaasgaard, S.; Olsen, J., 1994: Utilization of side-chain precursor for penicillin biosynthesis in a high-producing strain of Penicillium chrysogenum. Utilization of the side-chain precursors phenoxyacetic acid (POA) and phenylacetic acid (PA) for penicillin biosynthesis by Penicillium chrysogenum was studied in shake flasks. Precursor uptake and penicillin production were followed by HPLC analy...

Egen R.C.; Pathak S.G., 1980: Reduced utilization of side chain precursor for penicillin biosynthesis by mutants of penicillium chrysogenum. A high yielding strain of P. chrysogenum was mutated with ethylmethane sulfonate and investigated for selective effects of semicontinuous fermentations. Preferential growth of a class of mutants with different colony type and having reduced abilit...

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