Section 19
Chapter 18,707

Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)

Lau, L.-T.; Reid, S.M.; King, D.P.; Lau, A.M.-F.; Shaw, A.E.; Ferris, N.P.; Yu, A.C.-H.

Veterinary Microbiology 126(1-3): 101-110


ISSN/ISBN: 0378-1135
PMID: 17728080
DOI: 10.1016/j.vetmic.2007.07.008
Accession: 018706770

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A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n=43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV.

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