+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of a real-time PCR method for the potato-cyst nematode Globodera rostochiensis and the root-knot nematode Meloidogyne incognita



Development of a real-time PCR method for the potato-cyst nematode Globodera rostochiensis and the root-knot nematode Meloidogyne incognita



Soil Science and Plant Nutrition 54(1): 72-76



The primers PCN280f and NEPCN398r were designed for the quantitative detection of the potato-cyst nematode Globodera rostochiensis using real-time polymerase chain reaction (PCR). One, five, 50, 125 and 250 individuals of the second-stage juveniles (J2) of G. rostochiensis were mixed with various stages of vermiform Caenorhabditis elegans to make a total of 500 individuals and DNA was extracted from the nematode mixture. There was a significant correlation (r(2) = 0.9355, P < 0.001) between the threshold cycle values and the number of G. rostochiensis added. When nematodes were extracted from soils artificially infested with G. rostochiensis to various degrees and real-time PCR was conducted using DNA templates from the nematodes extracted, there was a highly significant correlation in the numbers of G. rostochiensis J2 from the real-time PCR method and morphological identification. Real-time PCR sensitively detected a single G. rostochiensis J2 out of 1,000 individuals of free-living nematodes. Similarly, real-time PCR primers RKNf and RKNr were designed for the detection of the root-knot nematode Meloidogyne incognita. This study demonstrated that the real-time PCR assay for the potato-cyst nematode and the root-knot nematode provides a sensitive and reliable means for the rapid quantification of these vermiform pests.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018720603

Download citation: RISBibTeXText

DOI: 10.1111/j.1747-0765.2007.00212.x


Related references

Control of potato cyst nematode globodera rostochiensis and root knot nematode meloidogyne incognita by organophosphorus carbamate benzimidazole and other compounds. Annals of Applied Biology 106(3): 489-498, 1985

The response of the potato cyst nematode, Heterodera rostochiensis, and the root knot nematode, Meloidogyne incognita to Dowco 275. Journal of Nematology 6(4): 136, 1974

Water uptake and hatching in the potato cyst nematode, Heterodera rostochiensis, and the root knot nematode, Meloidogyne incognita. Journal of Experimental Biology 61(3): 773-779, 1974

Study of the resistance of lines and hybrids of tomato to root knot nematodes (Meloidogyne sp.) and potato cyst nematode Globodera rostochiensis (Woll. 1923, Mulvey, Stone, 1976). Biotekhnologiya and Biotekhnika 2(1): 30-33, 1987

Multiplex real-time polymerase chain reaction for identifying potato cyst nematodes, Globodera pallida and Globodera rostochiensis, and the tobacco cyst nematode, Globodera tabacum. Canadian Journal Of Plant Pathology: 4, 554-564, 2008

Nature of resistance of cowpea to the root knot nematode meloidogyne incognita and the pigeon pea cyst nematode heterodera cajani. Indian Journal of Nematology 6(1): 81-85, 1976

Nature of resistance of cowpea to the root-knot nematode Meloidogyne incognita and to the pigeon pea cyst nematode, Heterodera cajani. Indian Journal of Nematology. 1976 ; 61: 81-85, 1978

Distribution of nematicides in soil and its influence on the control of sugar beet cyst nematode heterodera schachtii and root knot nematode meloidogyne incognita. Nematropica 12(2): 313-318, 1982

Protective effect of Dryopteris crassirhizoma extracts in the control of the root-knot nematode Meloidogyne incognitaol of the root-knot nematode Meloidogyne incognita. 2013

Detection and quantification of root-knot nematode (Meloidogyne javanica), lesion nematode (Pratylenchus zeae) and dagger nematode (Xiphinema elongatum) parasites of sugarcane using real-time PCR. Molecular and Cellular Probes 22(3): 168-176, 2008

Comparative proteomic analysis of the response of fibrous roots of nematode-resistant and -sensitive sweet potato cultivars to root-knot nematode Meloidogyne incognita. Acta Physiologiae Plantarum 39(12): 262, 2017

Pasteuria penetrans for controlling Meloidogyne incognita on sweet potato cultivars with different susceptibility to the root-knot nematode. II. Nematode-suppressive effects during the 7th to the 9th cropping seasons. Kyushu Plant Protection Research 47: 118-122, 2001

Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using Real-Time PCR with SYBR green I dye. Molecular and Cellular Probes 19(2): 81-86, 2005

Development of root knot nematode and morphometrics of females of the nematode Meloidogyne incognita, as influenced by different cucurbits. Indian Journal of Nematology 131: 79-83, 1983

Evaluation of nematicides for nematode control on field corn, 1981 Corn (Zea mays Pioneer 3369A), root lesion nematode; Pratylenchus spp., root-knot nematode; Meloidogyne incognita, ring nematode; Macroposthonia ornata. Fungicide and nematicide tests results American Phytopathological Society7(37): 190-191, 1982