+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Enzyme-linked immunosorbent assay for the detection of bovine rennet whey powder in milk powder and buttermilk powder



Enzyme-linked immunosorbent assay for the detection of bovine rennet whey powder in milk powder and buttermilk powder



International Dairy Journal 18(3): 294-302



An inhibition enzyme-linked immunosorbent assay (ELISA) for the detection of bovine rennet whey (BRW) solids in skim milk powders (SMP) and buttermilk powders is presented. The BRW content was determined in a neutralised trichloroacetic acid sample extract by binding of the dissolved caseinomacropeptide to an enzyme-labelled anti-bovine-K-casein monoclonal antibody. Calibration curves were constructed by analysing SMP standards with different known concentrations of BRW (0-5.8% (w/w)). The assay has a limit of detection of 0.1% (w/w) BRW powder in SMP and has high repeatability and reliability. The ELISA reached the sensitivity required for screening to conform with European Union (EU) legislation. It is easy to use, has a short assay time and is of low cost. The successful applicability of this new screening assay was demonstrated in comparison with chromatographic methods imposed by the EU, where 60 industrial samples taken by the Dutch General Inspection Service were analysed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018843007

Download citation: RISBibTeXText

DOI: 10.1016/j.idairyj.2007.08.008


Related references

Detection of rennet whey solids in skim milk powder and buttermilk powder with reversed phase hplc. Netherlands Milk & Dairy Journal 43(2): 171-184, 1989

Milk and milk powder, buttermilk and buttermilk powder, whey and whey powder: determination of phosphatase activity (routine method). IDF [International Dairy Federation] Standard (82: 1978 (Provisional)): 3, 1978

Milk and milk powder, buttermilk and buttermilk powder, whey and whey powder. Determination of phosphatase activity (routine method). Milchwissenschaft 34(4): 211-212, 1979

Milk and milk powder, buttermilk and buttermilk powder, whey and whey powder: determination of phosphatase activity (reference method). International Standard, International Dairy Federation (FIL-IDF 63: 1971): 5, 1971

Milk and dried milk, buttermilk and buttermilk powder, whey and whey powder. Detection of phosphatase activity (Screening method). International IDF Standard (82A: 1987 (Provisional)): 3, 1987

The detection of added whey powder in milk powder ii. the influence of residual amounts of lactose on the determination of whey powder in milk powder by means of the free sialic acid method. South African Journal of Dairy Science 18(3): 99-103, 1986

Milk and dried milk, buttermilk and buttermilk powder, whey and whey powder - determination of phosphatase activity (reference method). International Standard (ISO 3356-1975): 3, 1975

An enzyme-linked immunosorbent assay for monitoring of Aspergillus ochraceus growth in coffee powder, chilli powder and poultry feed. Letters in Applied Microbiology 42(1): 59-65, 2006

Determination of caseinomacropeptide with capillary zone electrophoresis and its application to the detection and estimation of rennet whey solids in milk and buttermilk powder. Electrophoresis 16(4): 529-533, 1995

Immunochromatographic lateral-flow test strip for the rapid detection of added bovine rennet whey in milk and milk powder. International Dairy Journal 19(4): 0-208, 2009

Detection of moulds in paprika powder by enzyme-linked immunosorbent assay. Acta Alimentaria 27(1): 97-103, 1998

Sensitivity and specificity enhanced enzyme-linked immunosorbent assay by rational hapten modification and heterogeneous antibody/coating antigen combinations for the detection of melamine in milk, milk powder and feed samples. Talanta 116: 173-180, 2013

Evaluation of enzyme-linked immunosorbent assay (ELISA) in milk powder contaminated with known concentrations of aflatoxin M1. Revista de Saude Publica 30(6): 542-548, 1996

Determination of vegetal proteins in milk powder by enzyme-linked immunosorbent assay: interlaboratory study. Journal of Aoac International 85(6): 1390-1397, 2002

Serum powder, buttermilk powder and milk powder. Technique laitiere: 30 (863) 13, 1975