EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Genetic screens for Caenorhabditis elegans mutants defective in left/right asymmetric neuronal fate specification


Genetic screens for Caenorhabditis elegans mutants defective in left/right asymmetric neuronal fate specification



Genetics 176(4): 2109-2130



ISSN/ISBN: 0016-6731

PMID: 17717195

DOI: 10.1534/genetics.107.075648

We describe here the results of genetic screens for Caenorhabditis elegans mutants in which a single neuronal fate decision is inappropriately executed. In wild-type animals, the two morphologically bilaterally symmetric gustatory neurons ASE left (ASEL) and ASE right (ASER) undergo a left/right asymmetric diversification in cell fate, manifested by the differential expression of a class of putative chemoreceptors and neuropeptides. Using single cell-specific gfp reporters and screening through a total of almost 120,000 haploid genomes, we isolated 161 mutants that define at least six different classes of mutant phenotypes in which ASEL/R fate is disrupted. Each mutant phenotypic class encompasses one to nine different complementation groups. Besides many alleles of 10 previously described genes, we have identified at least 16 novel "lsy" genes ("laterally symmetric"). Among mutations in known genes, we retrieved four alleles of the miRNA lsy-6 and a gain-of-function mutation in the 3'-UTR of a target of lsy-6, the cog-1 homeobox gene. Using newly found temperature-sensitive alleles of cog-1, we determined that a bistable feedback loop controlling ASEL vs. ASER fate, of which cog-1 is a component, is only transiently required to initiate but not to maintain ASEL and ASER fate. Taken together, our mutant screens identified a broad catalog of genes whose molecular characterization is expected to provide more insight into the complex genetic architecture of a left/right asymmetric neuronal cell fate decision.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 018991672

Download citation: RISBibTeXText

Related references

Nervous system-wide analysis of Hox regulation of terminal neuronal fate specification in Caenorhabditis elegans. Plos Genetics 18(2): E1010092, 2022

Mutations in the Caenorhabditis elegans gene vab-3 reveal distinct roles in fate specification and unequal cytokinesis in an asymmetric cell division. Developmental Biology 170(2): 679-689, 1995

Genetic analysis of endocytosis in Caenorhabditis elegans: coelomocyte uptake defective mutants. Genetics 159(1): 133-145, 2001

A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants. G3 11(4), 2021

Identification of genes that regulate a left-right asymmetric neuronal migration in Caenorhabditis elegans. Genetics 164(4): 1355-1367, 2003

A left/right asymmetric neuronal differentiation program is controlled by the Caenorhabditis elegans lsy-27 zinc-finger transcription factor. Genetics 188(3): 753-759, 2011

An unusual Zn-finger/FH2 domain protein controls a left/right asymmetric neuronal fate decision in C. elegans. Development 133(17): 3317-3328, 2006

The C. elegans Tailless/TLX transcription factor nhr-67 controls neuronal identity and left/right asymmetric fate diversification. Development 136(17): 2933-2944, 2009

Polarity and cell fate specification in the control of Caenorhabditis elegans gastrulation. Developmental Dynamics: An Official Publication of the American Association of Anatomists 238(4): 789-796, 2009

Cell fate specification and differentiation in the nervous system of Caenorhabditis elegans. Developmental Genetics 18(1): 73-80, 1996

Influences of HLH-2 stability on anchor cell fate specification during Caenorhabditis elegans gonadogenesis. G3 12(4), 2022

Signal transduction and cell fate specification during Caenorhabditis elegans vulval development. Current Opinion in Genetics and Development 4(4): 508-516, 1994

Male mating defective mutants of caenorhabditis elegans. Society for Neuroscience Abstracts 15(2): 1141, 1989

Chemotaxis-defective mutants of the nematode Caenorhabditis elegans. Genetics 80(2): 297-309, 1975

Hormesis in Caenorhabditis elegans dauer-defective mutants. Biogerontology 4(4): 203-214, 2003