+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Magnolol-induced H460 cells death via autophagy but not apoptosis

Magnolol-induced H460 cells death via autophagy but not apoptosis

Archives of Pharmacal Research (Seoul) 30(12): 1566-1574

We have reported that the protective effect of Magnolol on TBHP-induced injury in human non-small lung cancer H460 cells is partially via a p53 dependent mechanism. In this study, we found that Magnolol displayed a stimulatory effect at low concentrations (<= 20 mu M) whilst inhibitory effect at high concentrations (>= 40 mu M) in H460 cells. To investigate the mechanism of inducing the biphasic effect in H460 cells with Magnolol, we showed that Magnolol stimulated DNA synthesis at low concentrations and displayed an inhibition effect at high concentrations in H460 cells. More importantly, the inhibition of DNA synthesis was accompanied by the S phase cell cycle arrest and the appearance of intense intracytoplasmic vacuoles. These vacuoles can be labeled by autophagic marker monodansylcadaverin (MDC), 3-methyladenine (3MA), an inhibitor of autophagy, was able to inhibit the occurrence of autophagy. The results of the LDH activity assay and TUNEL assay also showed that Magnolol at high concentrations inhibiting H460 cell growth was not via apoptotic pathway. Furthermore, accompanied by the occurrence of autophagy, the expression of phospho-Akt was down-regulated but PTEN significantly was up-regulated. In conclusion, Magnolol induces H460 cells death by autophagy but not apoptotic pathway. Blockade of PI3K/PTEN/Akt pathway is maybe related to Magnolol-induced autophagy. Autophagic cells death induction by Magnolol underlines the potential utility of its induction as a new cancer treatment modality.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 019361703

Download citation: RISBibTeXText

Related references

Magnolol-induced H460 cells death via autophagy but not apoptosis. Archives of Pharmacal Research 30(12): 1566-1574, 2008

Protective effect of magnolol on TBHP-induced injury in H460 cells partially via a p53 dependent mechanism. Archives of Pharmacal Research 30(7): 850-857, 2007

Relative Apoptosis-inducing Potential of Homeopa-thic Condurango 6C and 30C in H460 Lung Cancer Cells In vitro: -Apoptosis-induction by homeopathic Condurango in H460 cells. Journal of Pharmacopuncture 17(1): 59-69, 2015

Induction of endoplasmic reticulum stress-mediated apoptosis and non-canonical autophagy by luteolin in NCI-H460 lung carcinoma cells. Food and Chemical Toxicology 56: 100-109, 2014

Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells. International Journal of Oncology 40(6): 1929-1940, 2012

Calyxin Y induces hydrogen peroxide-dependent autophagy and apoptosis via JNK activation in human non-small cell lung cancer NCI-H460 cells. Cancer Letters 340(1): 51-62, 2013

Magnolol induces apoptosis via activation of both mitochondrial and death receptor pathways in A375-S2 cells. Archives of Pharmacal Research 32(12): 1789-1794, 2010

Radiation-induced autophagy contributes to cell death and induces apoptosis partly in malignant glioma cells. Cancer Research and Treatment 47(2): 221-241, 2014

Antitumor effects of deguelin on H460 human lung cancer cells in vitro and in vivo: Roles of apoptotic cell death and H460 tumor xenografts model. Environmental Toxicology (): -, 2015

Polyamines modulate the roscovitine-induced cell death switch decision autophagy vs. apoptosis in MCF-7 and MDA-MB-231 breast cancer cells. Molecular Medicine Reports 11(6): 4532-4540, 2015

Gefitinib enhances human colon cancer cells to TRAIL-induced apoptosis of via autophagy- and JNK-mediated death receptors upregulation. Apoptosis 21(11): 1291-1301, 2016

Survival and death strategies in glioma cells: autophagy, senescence and apoptosis triggered by a single type of temozolomide-induced DNA damage. Plos One 8(1): E55665-E55665, 2013

Rottlerin induces autophagy and apoptotic cell death through a PKC-delta-independent pathway in HT1080 human fibrosarcoma cells: the protective role of autophagy in apoptosis. Autophagy 4(5): 650-658, 2008

ATP sensitizes H460 lung carcinoma cells to cisplatin-induced apoptosis. Chemico-Biological Interactions 184(3): 338-345, 2010

Upregulation of ATG3 contributes to autophagy induced by the detachment of intestinal epithelial cells from the extracellular matrix, but promotes autophagy-independent apoptosis of the attached cells. Autophagy 11(8): 1230-1246, 2016