EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Rapid identification of Candida glabrata in Candida bloodstream infections


Rapid identification of Candida glabrata in Candida bloodstream infections



Journal of Medical Microbiology 56(Part 12): 1639-1643



ISSN/ISBN: 0022-2615

PMID: 18033833

DOI: 10.1099/jmm.0.47406-0

Candida species are the fourth most common cause of bloodstream infection (BSI) in the hospitalized patient. Candida glabrata is the most common non-Candida albicans Candida species in England and Wales with an attributed mortality of 48%. C. glabrata is known to demonstrate reduced susceptibility to fluconazole, resulting in treatment failures when employing this agent for empirical treatment of Candida BSI. The first part of this study demonstrated a technique utilizing a blood culture system commonly used by many laboratories (BACTEC 9240 automated detection system) that reduced the time to identification of this potentially resistant organism by up to 72 h. A presumptive identification was achieved by observing a difference in the duration of incubation required before growth was detected automatically between Lytic Anaerobic and Plus Aerobic culture bottles. Secondly, experiments exploring the growth characteristics of C. glabrata in BACTEC blood culture bottles containing various media were carried out to explore possible reasons underpinning this clinical observation. The detection of yeast in the anaerobic bottle of a blood culture pair consisting of Lytic Anaerobic and Plus Aerobic in a BACTEC 9240 system was found to be highly predictive of the isolation of C. glabrata (positive predictive value 93.3%, negative predictive value 98.3%). The reason for this appeared to be a component of the Lytic Anaerobic blood culture medium enhancing the growth of C. glabrata in that medium.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 019846581

Download citation: RISBibTeXText

Related references

Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. Journal of Clinical Microbiology 34(1): 58-61, 1996

Comparison of costs, length of stay, and mortality associated with Candida glabrata and Candida albicans bloodstream infections. American Journal of Infection Control 38(1): 78-80, 2010

A multiplex PCR protocol for rapid identification of Candida glabrata and its phylogenetically related species Candida nivariensis and Candida bracarensis. Journal of Microbiological Methods 79(1): 117-120, 2009

Rapid identification of Candida albicans, Candida tropicalis, Candida glabrata, Cryptococcus neoformans by Fongiscreen 4H-R: Evaluation of 191 yeast strains. Journal de Mycologie Medicale 3(2): 103-106, 1993

Rapid, Presumptive Identification of Torulopsis (Candida) Glabrata and Candida Krusei Using Calcofluor White. Laboratory Medicine 38(4): 227-231, 2007

Rapid discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by use of a singleplex PCR. Journal of Clinical Microbiology 49(9): 3375-3379, 2011

Therapy and outcome of Candida glabrata versus Candida albicans bloodstream infection. Diagnostic Microbiology and Infectious Disease 60(3): 273-277, 2008

Initial treatment and outcome of Candida glabrata versus Candida albicans bloodstream infection. Diagnostic Microbiology and Infectious Disease 64(2): 152-157, 2009

Identification of Candida nivariensis and Candida bracarensis in a large global collection of Candida glabrata isolates: comparison to the literature. Journal of Clinical Microbiology 47(4): 1216-1217, 2009

A case of breakthrough Candida parapsilosis fungemia during micafungin therapy for a Candida glabrata bloodstream infection. Medical Mycology Case Reports 16: 1-3, 2017

Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections. Journal of Microbiological Methods 94(3): 262-266, 2013

Risk factors for fluconazole-resistant Candida glabrata bloodstream infections. Archives of Internal Medicine 169(4): 379-383, 2009

Activities of fluconazole and voriconazole against bloodstream isolates of Candida glabrata and Candida krusei: a 14-year study in a Spanish tertiary medical centre. International Journal of Antimicrobial Agents 31(3): 266-271, 2008

Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment. Journal of Clinical Microbiology 32(8): 1902-1907, 1994

Routine use of a commercial test, GLABRATA RTT, for rapid identification of Candida glabrata in six laboratories. Journal of Clinical Microbiology 42(10): 4870-4872, 2004