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Bone differentiation and formation using adipose tissue-derived stromal cells for cell-based therapies



Bone differentiation and formation using adipose tissue-derived stromal cells for cell-based therapies



Boei Ika Daigakko Zasshi 32(4): 186-197



Adipose tissue contains a population of cells with extensive self-renewal capacity and the ability to differentiate along multiple lineages. We examined whether adipose tissue appears to represent a potential for a clinically useful source of cells for tissue regeneration including bone. We compared the osteogenic differentiation of adipose tissue-derived stromal cells (ATSCs) with that of bone marrow-derived mesenchymal cells (BMCs). Optimal osteogenic differentiation in both cell types, as determined by secretion of osteocalcin and calcification, was obtained with the same three-dimensional culture. ATSCs had a similar ability to differentiate into osteoblasts to that of BMCs. Moreover, we found that a fraction adhered to the culture plate in a shorter time after cell seeding, demonstrating a novel potent capacity to differentiate osteoblasts in vitro and in vivo. We also established a method for culturing ATSCs using low-autologous serum to maintain their multilineage potential. Including these examinations, this review summarizes that ATSCs are useful for regeneration, osteogenic differentiation and bone formation.

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Accession: 020666930

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