+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Bone differentiation and formation using adipose tissue-derived stromal cells for cell-based therapies

Bone differentiation and formation using adipose tissue-derived stromal cells for cell-based therapies

Boei Ika Daigakko Zasshi 32(4): 186-197

Adipose tissue contains a population of cells with extensive self-renewal capacity and the ability to differentiate along multiple lineages. We examined whether adipose tissue appears to represent a potential for a clinically useful source of cells for tissue regeneration including bone. We compared the osteogenic differentiation of adipose tissue-derived stromal cells (ATSCs) with that of bone marrow-derived mesenchymal cells (BMCs). Optimal osteogenic differentiation in both cell types, as determined by secretion of osteocalcin and calcification, was obtained with the same three-dimensional culture. ATSCs had a similar ability to differentiate into osteoblasts to that of BMCs. Moreover, we found that a fraction adhered to the culture plate in a shorter time after cell seeding, demonstrating a novel potent capacity to differentiate osteoblasts in vitro and in vivo. We also established a method for culturing ATSCs using low-autologous serum to maintain their multilineage potential. Including these examinations, this review summarizes that ATSCs are useful for regeneration, osteogenic differentiation and bone formation.

(PDF emailed within 1 workday: $29.90)

Accession: 020666930

Download citation: RISBibTeXText

Related references

Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies. Stem Cells 25(4): 818-827, 2007

In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells. Veterinary Journal 191(2): 231-239, 2012

Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). CytoTherapy 15(6): 641-648, 2013

Fate of adipose-derived stromal vascular fraction cells after co-implantation with fat grafts: evidence of cell survival and differentiation in ischemic adipose tissue. Plastic and Reconstructive Surgery 132(2): 363-373, 2013

Human adipose-derived stromal cells for cell-based therapies in the treatment of systemic sclerosis. Cell Transplantation 22(5): 779-795, 2013

Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold. Journal of Biomedical Materials Research. Part B, Applied Biomaterials 76(1): 230-239, 2005

Comparison of immunological properties of bone marrow stromal cells and adipose tissue-derived stem cells before and after osteogenic differentiation in vitro. Tissue Engineering 13(1): 111-121, 2007

Volume-stable adipose tissue formation by implantation of human adipose-derived stromal cells using solid free-form fabrication-based polymer scaffolds. Annals of Plastic Surgery 70(1): 98-102, 2013

Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. Research in Veterinary Science 92(1): 66-75, 2012

Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. Veterinary Surgery 36(7): 613-622, 2007

Chemotaxis and differentiation of human adipose tissue CD34+/CD31- progenitor cells: role of stromal derived factor-1 released by adipose tissue capillary endothelial cells. Stem Cells 25(9): 2269-2276, 2007

Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo. Journal of Cellular and Molecular Medicine 9(4): 929-939, 2005

Pigment epithelial-derived factor and melanoma differentiation associated gene-7 cytokine gene therapies delivered by adipose-derived stromal/mesenchymal stem cells are effective in reducing prostate cancer cell growth. Stem Cells and Development 21(7): 1112-1123, 2012

Ectopic and in situ bone formation of adipose tissue-derived stromal cells in biphasic calcium phosphate nanocomposite. Journal of Biomedical Materials Research. Part A 81(4): 900-910, 2007

Adult human bone marrow- and adipose tissue-derived stromal cells support the formation of prevascular-like structures from endothelial cells in vitro. Tissue Engineering. Part A 16(1): 101-114, 2010