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Brilliant cresyl blue dye as a diagnostic tools for human malarial parasite in comparison to other known stains


Brilliant cresyl blue dye as a diagnostic tools for human malarial parasite in comparison to other known stains



Bulletin of Pure and Applied Sciences A Zoology uary- e; 20A(1): 9-16



Brilliant cresyl blue dye is (reticulocyte stain) was adapted in present study to identify malarial parasite stage in blood samples of confirmed malaria patients. The comparison of this stain with commonly used stains namely Giemsa's. Leishman's and JSB were carried out. Three main stages were observed during investigation by different stains viz. ring, schizont and gametocyte. Variations in the reliability of the stains to Plasmodium species as well as stages were obtained. Brilliant cresyl blue dye found to be a good stain for malaria parasite identification. The stain had shown higer reliability to ring and schizont stage, while its reliability to gametoeyte stage particularly for Plasmodium faiciparum was not reliable, hence the cytoplasm and the outer membrane of gametocyte remain unstained, which still need further study.

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