Cytological localization of -MSH, ACTH and -endorphin in the pars intermedia of the cichlid teleost Sarotherodon mossambicus

van Eys, GJJM. van den Oetelaar, P.

Cell and Tissue Research 2153: 625-633

1981


ISSN/ISBN: 0302-766X
DOI: 10.1007/bf00233536
Accession: 020803399

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Abstract
The pars intermedia of S. mossambicus contains 2 different endocrine-cell types. The predominant cell type is lead-hematoxylin-positive and assumed to synthesize MSH and related peptides. The 2nd cell type is PAS periodic acid-Schiff -positive, and its function and product(s) are unknown. Staining of light microscopic and ultrathin sections with antisera against .alpha.-MSH, ACTH 1-24 and human .beta.-endorphin revealed that only the lead-hematoxylin-positive cells of the pars intermedia react with these antisera, and that the secretory granules of these cells contain compounds that were immunoreactive to all 3 antisera. .alpha.-MSH, ACTH and endorphins may be derived from the same precursor molecule. No specific reaction with 1 of the antisera was detected in the PAS-positive cells.

Cytological localization of -MSH, ACTH and -endorphin in the pars intermedia of the cichlid teleost Sarotherodon mossambicus