+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Determination of genes involved in the early process of molar root development initiation in rat by modified subtractive hybridization



Determination of genes involved in the early process of molar root development initiation in rat by modified subtractive hybridization



Biochemical and Biophysical Research Communications 363(4): 994-1000



Rat molars and incisors have different root patterning mechanisms: the former form a multirooted pattern while the latter form a single-rooted analogue. But the genetic signals that control root patterning are poorly understood. In this study, to identify the special molecular signals which may lead to the molar root development, we firstly observed that at embryonic day 19 the molar and the incisor began differentially developing: the molar formed double-layered cells of the root sheath while the incisor formed a cervical loop. By using the subtractive hybridization method, we successfully constructed a subtraction cDNA library of the rat molar and incisor tissues. Differentially expressed gene clones were evaluated by dot-blot and sequencing. Sel1l, Nfic, Edar, GATA6, and some novel genes were found differentially expressed, which were strongly related the tooth root patterning. It is anticipated that further study of genes identified will provide insights into their specific roles in the tooth root patterning.

(PDF emailed within 0-6 h: $19.90)

Accession: 020836622

Download citation: RISBibTeXText

PMID: 17923110

DOI: 10.1016/j.bbrc.2007.09.087


Related references

Determination of genes involved in the early process of embryonic implantation in rhesus monkey (Macaca mulatta) by suppression subtractive hybridization. Biology of Reproduction 70(5): 1365-1373, 2004

Identification of genes involved in indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.) by suppression subtractive hybridization. Gene 514(2): 91-98, 2013

Identification of early salt stress response genes in tomato root by suppression subtractive hybridization and microarray analysis. Journal of Experimental Botany 58(3): 507-520, 2007

Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development. Molecular Plant-Microbe Interactions 9(4): 233-242, 1996

Determination of genes expressed during early stages of regeneration using subtractive hybridization in an echinoderm model system. Abstracts of the General Meeting of the American Society for Microbiology 99: 672-673, 1999

Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH). Genetics and Molecular Biology 33(4): 676-685, 2011

Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton. Molekuliarnaia Biologiia 40(5): 825-834, 2006

Isolation of genes involved in pancreas regeneration by subtractive hybridization. Biological Chemistry 391(9): 1019-1029, 2011

Use of suppression subtractive hybridization approach to identify genes differentially expressed during early banana fruit development undergoing changes in ethylene responsiveness. Plant Science (Oxford) 172(5): 1025-1036, 2007

Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization. Human and Experimental Toxicology 34(1): 3-11, 2015

Modified suppression subtractive hybridization identifies an AP2-containing protein involved in metal responses in Physcomitrella patens. Molecules and Cells 23(1): 100-107, 2007

Identification of Differential Genes by Suppression Subtractive Hybridization: II. Subtractive Hybridization. Csh Protocols 2008: Pdb.Prot4856, 2008

Search for genes involved in developmental competence in mouse oocytes using suppression subtractive hybridization. Reproduction Fertility & Development 16(1-2): 245-246, 2004

EST analysis of genes involved in secondary metabolism in Camellia sinensis (tea), using suppression subtractive hybridization. Plant Science 166(4): 953-961, 2004

Identification of genes involved in color variation of bamboo culms by suppression subtractive hybridization. Plant Physiology and Biochemistry 97: 156-164, 2016