+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis



Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis



Journal of Medical Microbiology 56(Pt 11): 1467-1473



A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 020840742

Download citation: RISBibTeXText

PMID: 17965346

DOI: 10.1099/jmm.0.47363-0


Related references

Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay. Journal of Clinical Microbiology 37(6): 1790-1796, 1999

Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA. Journal of Clinical Microbiology 42(12): 5549-5557, 2004

Molecular discrimination between Campylobacter jejuni, Campylobacter coli, Campylobacter lari and Campylobacter upsaliensis by polymerase chain reaction based on a novel putative GTPase gene. Molecular and Cellular Probes 11(3): 177-185, 1997

A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari. Japanese Journal of Infectious Diseases 69(3): 256-258, 2017

Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus. Fems Immunology and Medical Microbiology 52(2): 260-266, 2008

Differentiation of campylobacter jejuni campylobacter coli campylobacter lari and campylobacter fetus based on the parietal fatty acids profiles obtained by gas liquid chromatography mass spectrometry as well as on the hippurate hydrolysis. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene 83(2): 142-150, 1992

Simultaneous Identification of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari with SmartCycler-Based Multiplex Quantitative Polymerase Chain Reaction. Foodborne Pathogens and Disease 14(7): 371-378, 2017

A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis. Journal of Medical Microbiology 63(Pt 5): 659-666, 2014

Pulsed-field gel electrophoretic discrimination of Campylobacter lari from two other thermophilic species of Campylobacter (Campylobacter coli and Campylobacter jejuni). Physico Chemical Biology 37(2): 63-67, 1993

Rapid detection and differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in food, using multiplex real-time PCR. Journal of Food Protection 73(2): 241-250, 2010

Rapid Detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in Fresh Chicken Meat and By-Products in Bangkok, Thailand, Using Modified Multiplex PCR. Journal of Food Protection 78(7): 1363-1369, 2016

The role of Campylobacter sputorum subspecies mucosalis, Campylobacter hyointestinalis and Campylobacter coli in the etiology of porcine proliferative enteritis. Dissertation Abstracts International, B 44(12): 3678, 1984

Identification of Campylobacter jejuni, Campylobacter coli and campylobacter lari by the nucleic acid amplification system NASBAR. Journal of Applied Bacteriology 77(6): 694-701, 1994

Identification of Campylobacter jejuni, Campylobacter coli and Campylobacter lari by the nucleic acid amplification system NASBR. Journal of Applied Microbiology 77(6): 694-701, 1994

Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. Journal of Clinical Microbiology 40(12): 4744-4747, 2002