Discrimination of indica and japonica subspecies and variations of vascular bundle characteristics in recombinant inbred lines derived from an indica/japonica cross

Zhu-Chun-Jie; Xu-Hai; Guo-Yan-Hua; Wang-Jia-Yu; Liu-Hong-Guang; Xu-Zheng-Jin

Zhongguo Shuidao Kexue 21(6): 619-624

2007


Accession: 020871815

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Abstract
A recombinant inbred line (RIL,) population derived from a cross between Qishanzhan (typical indica) and Akihikari atypical japonica) was used as materials. The result of subspecies discrimination in F-6 and F-8 generations based on the Cheng's index and vascular bundle characteristics showed that intermediate type lines were dominant and both populations were japonicalinous. The vascular bundle characteristics which were significantly different between indica and japonica were recombined in the RIL population. The discrimination results based on the ratio of large to small vascular bundles. in particle neck (RLSVB) and the ratio of large vascular bundles between the second internode from the top and panicle neck (RLVB) were consisted with those by the Cheng's index, so they could be regarded as the parameters for subspecies classification. Moreover, these parameters were more stable than the number of large or small vascular bundles in panicle neck or the second internode from the top among different generations. Because most lines in the populations were intermediate type without complete indica and japonic a differentiation, the discrimination results in a certain line based on RLSVB and RLVB might be different.