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Distribution of SHV genotypes and resistance analysis of extended-spectrum beta-lactamases-producing klebsiella pneumoniae and escherichia coli isolates in Hefei



Distribution of SHV genotypes and resistance analysis of extended-spectrum beta-lactamases-producing klebsiella pneumoniae and escherichia coli isolates in Hefei



Zhongguo Yaolixue Tongbao 23(6): 812-815



Aim To study the SHV genotype and analyze resistance of extended-speetru in beta-lactamases ESBLs) -producing Klebsiella pneumoniae (Kpn and Escherichia coli (Eco) strains isolated from clinical patients in Hefei during 1999 similar to 2000. Methods Ninety-eight strains of Klebsiella pneumoniae and Escherichia coll conserved by Anhui center for surveilance of bacterial resistance were collected from four general hospitals, including First affiliated hospital of Anhui medical university, Anhui province hospital, first people's hospital of Hefei and the 105th hospital of PLA, from September 1999 to September 2000. The encoding genes of SHV beta-lactamases produced by ESBLs-producing isolates were amplified by PCR. The purified PCR products were cloned into pGEM-Teasy vectors and sequenced by sanger's dideoxy chain termination composition method recommended by CLS1. Their resistances to I I antimicrobial agents were tested by agar dilution. MICs of the antimicrobial agents against clinical isolates and the transconjugants were determined. Results Twenty-nine out of ninety-eight ESBLs-producing isolates produced SHV beta-lactamases. Of twenty-nine isolates producing SHV beta-lactamases, eight strains produced SHV-1, five strains produced SHV-1 I, eleven strains produced SHV-12, one strain produced SHV-18, one strain produced SHV-28, and three produced a novel SHV type SHV-89 (Accession No. DQ193536). All the strains had the same susceptible pattern, with the low susceptibility rate to piperacillin, ceftriaxone and cefotaxime. All the strains were susceptible to imipenem. Compared with clinical strains, the transconjugants showed higher resistance to many antimicrobial agents. Conclusions The rates of SHV beta-lactamases-producing klebstella. pneumoniae and Escherichia coli reach a high level in hospitals in Hefei. This is the first report on SHV-89 in China. The high resistance may be present in these clinical isolates in Hefei area.

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