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Effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene on hematopoietic stem cells mobilization in patients with extensive coronary heart disease



Effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene on hematopoietic stem cells mobilization in patients with extensive coronary heart disease



Zhonghua Xin Xue Guan Bing Za Zhi 35(6): 504-508



Objective To investigate the effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene (Ad(5)-HGF) on hematopoietic stem cells mobilization in patients with extensive coronary heart disease. Methods Patients with extensive coronary heart disease were treated with intracoronary infusion of adenovirus vector encoding hepatocyte growth factor (Ad(5)-HGF 5 x 10(9) pfu) gene plus stent implantation (n=9) or equal physiological saline plus stent implantation (n=9). Angioplasty and stent implantation was performed according to standard clinical practice by the femoral approach and blood samples were drawn from each patient at baseline before PCI, 6 to 24 hours and 6 days post procedure. The number of CD34(+) , CD38(+) and CD117(+) cells in peripheral blood was analyzed by flow cytometer. Results The number of circulating CD34(+) cells in Ad, -HGF gene treatment group 6 hours after procedure and the number of circulating CD117(+) cells 6 days post procedure were significantly higher in Ad, -HGF gene treatment group than those in the control group 0.104 +/- 0.082 vs. 0.022 +/- 0.012, P = 0.021) and (0.058 +/- 0.058 vs. 0.012 +/- 0.009, P = 0. 034 respectively. Conclusion Intracoronary administration of Ad(5) -HCF could mobilize hematopoietic stem cells into peripheral blood and the consequent rote of this observation on myocardial regeneration warrants further detailed studies.

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Accession: 020923012

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PMID: 17711706


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