+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

FMRFamide- and pancreatic polypeptide-like immunoreactivity of endocrine cells in the midgut of a mosquito

FMRFamide- and pancreatic polypeptide-like immunoreactivity of endocrine cells in the midgut of a mosquito

Tissue & Cell 18(3): 419-428

Immunocytochemical surveys of midguts from female mosquitoes, Aedes aegypti, reveal that half of the estimated 500 endocrine cells in a midgut contain a substance recognized by antisera to bovine pancreatic polypeptide and a molluscan peptide. FMRF-amide (phenylalanine-methionine-arginine-phenylalanine-amide). With light microscopy the cells resemble an endocrine type because of their basal position in the epithelium, conical shape, and, in some instances, apical extensions to the lumen. At the ultrastructural level, the immunoreactive substance is contained specifically within the secretory granules of such cells. Immunoreactive cells are distributed exclusively in the midgut region where blood is stored, and ingestion of vertebrate blood reduces the number of such cells and the intensity of reaction in others. These two facts suggest that a blood meal stimulates release of the immunoreactive substance from the cells. Since the immunocytochemical localization is supplemented by a demonstrated secretory response, the cells are considered to be peptidergic endocrine cells.

(PDF same-day service: $19.90)

Accession: 021018073

Download citation: RISBibTeXText

PMID: 3738889

DOI: 10.1016/0040-8166(86)90061-3

Related references

Phenylalanylmethionylarginylphenylalaninamide like and pancreatic polypeptide like immunoreactivity of endocrine cells in the midgut of a mosquito aedes aegypti. Tissue & Cell 18(3): 419-428, 1986

Coexistence of pancreatic polypeptide (PP)-and glucagon-immunoreactivity in pancreatic endocrine cells of mouse. Histochemistry 87(1): 1-6, 1987

Immunocytochemical and ultrastructural characterization of coexistence of pancreatic polypeptide and glucagon-like immunoreactivity in the pancreatic endocrine cells of Sparus auratus L. (Teleostei). General and Comparative Endocrinology 70(1): 9-19, 1988

Ultrastructural and immunohistochemical identification of pancreatic polypeptide-immunoreactive endocrine cells in the cockroach midgut. Biomedical Research 3(4): 454-456, 1982

The distribution of bovine pancreatic polypeptide/FMRFamide-like immunoreactivity in the ventral nervous system of the locust. Journal of Comparative Neurology 234(1): 1-16, 1985

Immunocytochemically demonstrable icosapeptide fragment of the pancreatic polypeptide precursor presence in rectal but absence from pancreatic endocrine tumors containing pancreatic polypeptide immunoreactive cells. Digestive Diseases & Sciences 29(8 SUPPL): 85S, 1984

FMRFamide-like midgut endocrine cells during the metamorphosis in Melipona quadrifasciata anthidioides (Hymenoptera, Apidae). Micron 33(5): 453-460, 2002

FMRFamide-like immunoreactive midgut endocrine cells in different castes of the bee Melipona quadrifasciata anthidioides (Apidae; Meliponini). Brazilian Journal of Morphological Sciences 20(3): 157-164, 2003

An identified dorsal unpaired median neurone and bilaterally projecting neurones exhibiting bovine pancreatic polypeptide-like/FMRFamide-like immunoreactivity in abdominal ganglia of the migratory locust. Cell and Tissue Research 267(1): 85-98, 1992

Feeding state influences the content of FMRFamide- and tachykinin-related peptides in endocrine-like cells of the midgut of Locusta migratoria. Peptides 22(2): 229-234, 2001

Islet amyloid polypeptide and calcitonin gene-related peptide immunoreactivity in amyloid and tumor cells of canine pancreatic endocrine tumors. Veterinary Pathology 27(3): 194-198, 1990

Localization of FMRFamide-like immunoreactivity in the larval midgut of the fly, Sarcophaga bullata. Comparative biochemistry and physiology: C: Comparative pharmacology and toxicology 102(3): 555-560, 1992

Human pancreatic polypeptide (HPP) immunoreactivity in an infiltrating endocrine tumour of the papilla of Vater with unusual morphology. Virchows Archiv. A, Pathological Anatomy and Histology 392(1): 119-126, 1981