+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates



Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates



Chromosoma 117(2): 181-187



Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)(8) (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 021052268

Download citation: RISBibTeXText

PMID: 18046569

DOI: 10.1007/s00412-007-0136-2


Related references

Direct fluorescence in situ hybridization on human metaphase chromosomes using quantum dot-platinum labeled DNA probes. Biochemical and Biophysical Research Communications 467(2): 328-333, 2015

Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes. Methods in Molecular Medicine 53: 101-123, 2001

Detection of aneuploidy involving chromosomes 13, 18, or 21, by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes. American Journal of Human Genetics 49(1): 112-119, 1991

Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH). Chromosome Research 4(1): 24-28, 1996

Cytogenetic analysis of metaphase chromosomes from pupal testes of four mosquito species using fluorescence in situ hybridization technique (FISH). World Journal of Microbiology and Biotechnology 21(4): 515-518, 2005

Fluorescence in situ hybridization on metaphase chromosomes with biotinylated probes. In situ hybridization, biotin labeling, cosmids, gene mapping, oncogene amplification. Nouvelle Revue Francaise d'Hematologie 32(6): 459-460, 1990

The latest achievements of radiation cytogenetics in connection with the discovery of the method for the fluorescence hybridization in situ of the metaphase chromosomes of man and experimental animals using DNA probes (FISH). Tsitologiia i Genetika 30(4): 70-85, 1996

Fluorescence in situ hybridization (FISH): Detection of biotin- and digoxigenin-labeled signals on chromosomes. Journal of Tissue Culture Methods 16(1): 39-47, 1994

Fluorescence in situ hybridization (FISH) on human chromosomes using photoprobe biotin-labeled probes. Journal of Histochemistry and Cytochemistry 51(4): 549-551, 2003

Quantum-dot-labeled DNA probes for fluorescence in situ hybridization (FISH) in the microorganism Escherichia coli. Chemphyschem 7(5): 1062-1067, 2006

Identification of parental chromosomes and changes of artificial, inter-generic F-1 hybrid between Dendranthema horaimontana and Nipponanthemum nipponicum by fluorescence genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). Chromosome Science 8(3): 71-79, 2004

Fluorescence in situ hybridization of metaphase chromosomes in suspension. International Journal of Radiation Biology 77(7): 787-795, 2001

Fluorescence in situ hybridization to metaphase and interphase chromosomes. Cancer Genetics & Cytogenetics 66(2): 141, 1993

Atypical cat eye syndrome. Fluorescence in situ hybridization of metaphase chromosomes. Monatsschrift Kinderheilkunde 140(8): 460-463, 1992

Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization. Cold Spring Harbor Symposia on Quantitative Biology 58: 767-775, 1993