+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Impairing and monitoring glucose catabolite repression in L-carnitine biosynthesis

Impairing and monitoring glucose catabolite repression in L-carnitine biosynthesis

Biotechnology Progress 23(6): 1286-1296

Signal transduction pathways are usually avoided when optimizing a biotransformation process because they require complex mathematical formulations. The aim of this work was to use a Systems Biology approach to optimize and monitor the biotransformation of L-carnitine using signal transduction pathways. To this end, a dynamic model was constructed, integrating the metabolic pathways of L-carnitine biosynthesis as well as the expression of this metabolism by means of its regulation by transcription factors such as cAMP-CRP and CaiF. The model was validated using different C-sources as well as different reactor feeding approaches. A linear relationship between the external cellular cAMP and the L-carnitine production levels was predicted before being experimentally confirmed in several scenarios. Moreover, results of the model simulations and subsequent experimental findings demonstrated that the addition of exogenous cAMP was able to restore the L-carnitine production when glucose was used as C-source. Additionally, a way to monitor the L-carnitine biosynthesis by using the level of cAMP as a marker of the biotransformation state was in silico and experimentally demonstrated.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 021172591

Download citation: RISBibTeXText

PMID: 18062670

DOI: 10.1021/bp070213t

Related references

Catabolite repression of glucose isomerase biosynthesis in streptomyces albogriseolus. Biotekhnologiya 4(4): 457-462, 1988

Regulation of the biosynthesis of the antibiotic indolmycin possible catabolite repression by glucose. Lloydia (cincinnati): 470, 1972

Glucose catabolite repression of glucoamylase biosynthesis in the yeast Endomycopsis fibuligera. Applied biochemistry and microbiology (pub l1979) 14(6): 676-681, 1979

Glucose catabolite repression of gluco amylase biosynthesis by the yeast endomycopsis fibuliger. Prikladnaya Biokhimiya i Mikrobiologiya 14(6): 878-884, 1978

Regulation of secondary metabolite biosynthesis: catabolite repression of phenoxazinone synthase and actinomycin formation by glucose. Journal of Bacteriology 109(2): 659-667, 1972

Effect of cell density and glucose uptake on catabolite repression in a catabolite insensitive mutant of escherichia coli strain b r. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 178, 1974

Parallel changes in catabolite repression of haem biosynthesis and cytochromes in repression-resistant mutants of Saccharomyces cerevisiae. Journal of General Microbiology 135(5): 1217-1227, 1989

Cyclid AMP and catabolite repression in yeasts, In Schizosaccharomyces pombe glucose lowers both intracellular adenosine 3':5'-monophosphate levels and the activity of catabolite-sensitive enzymes. European Journal of Biochemistry 49(1): 305-316, 1974

A reporter gene analysis of penicillin biosynthesis gene expression in Penicillium chrysogenum and its regulation by nitrogen and glucose catabolite repression. Applied and Environmental Microbiology 60(12): 4432-4439, 1994

Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of unrelated sequence, and glucose exerts additional xylR-dependent repression. Journal of Bacteriology 176(6): 1738-1745, 1994

Carbon catabolite repression of sucrose utilization in Staphylococcus xylosus: catabolite control protein CcpA ensures glucose preference and autoregulatory limitation of sucrose utilization. Journal of Molecular Microbiology and Biotechnology 12(1-2): 114-120, 2007

Catabolite repression of protoporhyrin IX biosynthesis in Escherichia coli K-12. Febs Letters 62(3): 351-353, 1976

Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus. Journal Of Basic Microbiology. 34(6): 371-378, 1994

Catabolite repression of L-asparaginase biosynthesis in Bacillus mesentericus. Mikrobiologiia 43(5): 825-829, 1974

PHB biosynthesis in catabolite repression mutant of Burkholderia sacchari. Current Microbiology 63(4): 319-326, 2011