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Methodes simplifiees de recolte des formes larvaires de Wuchereria bancrofti Cobbard (var. pacifica): leur preparation pour immunofluorescence



Methodes simplifiees de recolte des formes larvaires de Wuchereria bancrofti Cobbard (var. pacifica): leur preparation pour immunofluorescence



Bulletin of the World Health Organization 562: 305-308



To provide antigenic material in sufficient quantities for immunological studies, a simple technique was developed for extracting infective stage larvae of W. bancrofti from Aedes polynesiensis raised in an insectary. Experimentally infected mosquitos were caught and after anesthetization were held under physiological saline solution for 12-24 h. In this time the infective larvae emerged from the mosquitos and could be collected. The suspension of larvae was then introduced under slight pressure into a short sealed section of rat thoracic artery to obtain homogeneous distribution of the larvae in the artery walls. Frozen sections of the artery were then suitable for immunofluorescence tests. For the collection of microfilariae, a modification of Sawyer & Weinstein's method was used. Blood from a highly parasitized human subject was collected in a citrated bottle, and, after lysis of the red cells with saponin, was centrifuged. Treatment of the sediment with streptolysin then released the living microfilariae, which when taken up in physiological saline solution were ready for use in immunofluorescence tests.

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