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Nucleoside diphosphate kinase from Artemia embryos



Nucleoside diphosphate kinase from Artemia embryos



Comparative Biochemistry and Physiology B Comparative Biochemistry 781: 141-144



1.1. Nucleoside diphosphate kinase (EC. 2.7.4.6) has been purified 1900-fold with 17% yield from Artemia dormant embryos.2.2. The purified enzyme exhibits a ping-pong mechanism of reaction. The calculated Km values for ADP, GTP, ITP, UTP and CTP are 0.04, 0.17, 0.25, 0.27 and 0.62 mM, respectively.3.3. The role of this enzyme in the conversion of diguanosine tetraphosphate into ATP is also discussed. Maximum velocity of nucleoside diphosphate kinase (50 U/g dry cysts) is well in excess (around thousand-fold) over the enzymes which transform diguanosine tetraphosphate into AMP.

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Accession: 021450277

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DOI: 10.1016/0305-0491(84)90157-3


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