+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

On the specificity of uptake by isolated Torpedo synaptic vesicles



On the specificity of uptake by isolated Torpedo synaptic vesicles



Neuroscience Letters 23(1): 81-85



Synaptic vesicles were isolated from the electric organ of Torpedo marmorata in highly purified form. Their uptake properties were examined using a large number of small organic molecules as substrates. Following incubation at 26 degree C for 1 h, it was found that concentrative accumulation, indicated by a vesicle:medium concentration ratio greater than unity, was achieved by all the choline analogues used and by four biogenic amines, but not by a variety of purine and pyrimidine bases and nucleosides. Amino acids penetrated poorly, as did sugars, and of organic anions, acetate but not citrate or thiocyanate, was almost excluded. Thus Torpedo vesicles are relatively impermeable to compounds which cannot utilize the ACh or ATP carriers, but show a very high rate of amine uptake, which may be linked to a pH gradient.

(PDF emailed within 0-6 h: $19.90)

Accession: 021491267

Download citation: RISBibTeXText

PMID: 7231816

DOI: 10.1016/0304-3940(81)90191-9


Related references

Specificity of uptake by isolated torpedo marmorata synaptic vesicles. Neuroscience Letters 23(1): 81-86, 1981

Uptake system for acetylcholine in isolated torpedo synaptic vesicles and its pharmacology. Dowdall, M J And J N Hawthorne (Ed ) Ellis Horwood Series in Biomedicine: Cellular And Molecular Basis Of Cholinergic Function; Meeting, Buxton, England, Uk, May 1986 Xviii+941p Ellis Horwood Ltd : Chichester, England, Uk; Vch Verlagsgesellschaft: Weinheim, West Germany (Dist in Usa And Canada By Vch Publishers, New York, New York, Usa) Illus 303-315, 1987

ATP-dependent calcium uptake by cholinergic synaptic vesicles isolated from Torpedo electric organ. Journal of Membrane Biology 54(2): 115-126, 1980

AH5183 and cetiedil: two potent inhibitors of acetylcholine uptake into isolated synaptic vesicles from Torpedo marmorata. Journal of Neurochemistry 52(3): 813-821, 1989

Cholinergic synaptic vesicles isolated from Torpedo marmorata: demonstration of acetylcholine and choline uptake in an in vitro system. Neuroscience 5(6): 1041-1052, 1980

Uptake of acetylcholine into Torpedo synaptic vesicles in vitro. Advances in Behavioral Biology, 25: 301-306, 1981

Specificity of association of a Ca2+/Mg2+ ATPase with cholinergic synaptic vesicles from Torpedo electric organ. Biochemical and Biophysical Research Communications 88(3): 1069-1076, 1979

The role of thiols in nucleotide uptake into synaptic vesicles from Torpedo marmorata. European Journal of Biochemistry 166(3): 553-558, 1987

Specific stimulated uptake of acetylcholine by Torpedo electric organ synaptic vesicles. Proceedings of the National Academy of Sciences of the United States of America 77(10): 6234-6238, 1980

Specificity of association of a calcium magnesium atpase with cholinergic synaptic vesicles from torpedo californica electric organ. Biochemical & Biophysical Research Communications 88(3): 1069-1076, 1979

Passive uptake of acetylcholine and other organic cations by synaptic vesicles from Torpedo electric organ. Biochemistry 19(18): 4373-4379, 1980

Structure and stoichiometry of isolated torpedo electric organ synaptic vesicles. Biophysical Journal 21(3): 53A, 1978

Bicarbonate and magnesium ion-ATP dependent stimulation of acetylcholine uptake by Torpedo electric organ synaptic vesicles. Biochemical and Biophysical Research Communications 94(1): 305-312, 1980

Proton gradient linkage to active uptake of (3H) acetylcholine by Torpedo electric organ synaptic vesicles. Biochemistry, 2113: 3037-3043, 1982

A morphometric analysis of isolated Torpedo electric organ synaptic vesicles following stimulation. Brain Research 525(1): 59-70, 1990