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On the structures of filamentous bacteriophage Ff (fd, f1, M13)



On the structures of filamentous bacteriophage Ff (fd, f1, M13)



European Biophysics Journal 37(4): 521-527



The filamentous bacteriophage (Inovirus) strain Ff (fd, f1, M13) is widely used in molecular biophysics as a simple model system. A low resolution molecular model of the fd protein coat has been reported, derived from iterative helical real space reconstruction of cryo-electron micrographs (cryoEM). This model is significantly different from the model previously derived from X-ray fibre diffraction and solid-state NMR. We show that the cryoEM model agrees neither with solid-state NMR data nor with X-ray fibre diffraction data of fd, and has some puzzling structural features, for instance nanometre holes through the protein coat. We refine the cryoEM model against the X-ray data, and find that the model after refinement closely approximates the model derived directly from X-ray fibre diffraction and solid-state NMR data. We suggest possible reasons for the differences between the models derived from cryoEM and X-ray diffraction.

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Accession: 021491690

Download citation: RISBibTeXText

PMID: 17943277

DOI: 10.1007/s00249-007-0222-7


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