+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Organic and inorganic selenium speciation in environmental and biological samples by nanometer-sized materials packed dual-column separation/preconcentration on-line coupled with ICP-MS



Organic and inorganic selenium speciation in environmental and biological samples by nanometer-sized materials packed dual-column separation/preconcentration on-line coupled with ICP-MS



Journal of Mass Spectrometry 43(3): 336-345



A novel, fast, and cheap nonchromatographic method for direct speciation of dissolved inorganic and organic selenium species in environmental and biological samples was developed by flow injection (FI) dual-column preconcentration/separation on-line coupled with ICP-MS determination. In the developed technique, the first column packed with nanometer-sized Al(2)O(3) could selectively adsorb the inorganic selenium [Se(IV), Se(VI)], and the retained inorganic selenium could be eluted by 0.2 mol l(-1) NaOH, while the organic Se [selenocystine (SeCys(2)) and selenomethionine (Se-Met)] was not retained. On the other hand, the second column packed with mesoporous TiO(2) chemically modified by dimercaptosuccinic acid (DMSA) could selectively adsorb Se(IV) and SeCys(2) and barely adsorb Se(VI) and Se-Met. When the sample solution was passed through the column 1, separation of inorganic selenium and organic selenium could be achieved first. Then, the effluent from column 1 was successively introduced into the column 2 and the speciation of organic selenium could be attained due to the different adsorption behaviors of Se-Met and SeCys(2) on DMSA modified TiO(2). After that, the eluent from column 1 contained Se(IV), and Se(VI) was adjusted to desired pH and injected into column 2, and the speciation of Se(IV) and Se(VI) could also be realized thanks to their different retention on column 2. The parameters affecting the separation were investigated systematically and the optimal separation conditions were established. The detection limits obtained for Se(IV), Se(VI), Se-Met and SeCys(2) were 45-210 ng l(-1) with precisions of 3.6-9.7%. The proposed method has been successfully applied for the speciation of dissolved inorganic and organic selenium in environmental and biological samples. In order to validate the methodology, the developed method was also applied to the speciation of selenium in certified reference material of SELM-1 yeast, and the determined values were in good agreement with the certified values.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 021501050

Download citation: RISBibTeXText

PMID: 17994643

DOI: 10.1002/jms.1321


Related references

Simultaneous on-line preconcentration and determination of trace metals in environmental samples by flow injection combined with inductively coupled plasma mass spectrometry using a nanometer-sized alumina packed micro-column. Analytica Chimica Acta 540(2): 333-339, 2005

Simultaneous on-line preconcentration and determination of trace metals in environmental samples using a modified nanometer-sized alumina packed micro-column by flow injection combined with ICP-OES. Talanta 71(3): 1239-1245, 2007

Nanometer-sized alumina packed microcolumn solid-phase extraction combined with field-amplified sample stacking-capillary electrophoresis for the speciation analysis of inorganic selenium in environmental water samples. Electrophoresis 33(19-20): 2953-2960, 2013

Nanometer-sized zirconium dioxide microcolumn separation/preconcentration of trace metals and their determination by ICP-OES in environmental and biological samples. Microchimica Acta 159(3-4): 379-385, 2007

On-Line Separation and Preconcentration of Trace Metals in Biological Samples Using a Microcolumn Loaded with PAN-Modified Nanometer-Sized Titanium Dioxide, and Their Determination by ICP-AES. Microchimica Acta 144(4): 227-231, 2004

Micro-column preconcentration/separation using thiacalix arene tetracarboxylate derivative modified mesoporous TiO2 as packing materials on-line coupled to inductively coupled plasma optical emission spectrometry for the determination of trace heavy metals in environmental water samples. Microchemical Journal: 1, 90-95, 2010

On-line separation and preconcentration of trace cadmium in environmental water samples by micro column filled with modified nanometer Si-HAP prior to FAAS determination. International Journal of Environmental & Analytical Chemistry 92(7): 821-831, 2012

Speciation of dissolved iron(II) and iron(III) in environmental water samples by gallic acid-modified nanometer-sized alumina micro-column separation and ICP-MS determination. Analyst 130(8): 1175-1181, 2005

Speciation of inorganic selenium in environmental water samples by inductively coupled plasma optical emission spectrometry after preconcentration by using a mesoporous zirconia coating on coal cinder. Journal of Separation Science 37(16): 2260-2267, 2015

Speciation analysis of chromium in natural water samples by electrothermal atomic absorbance spectrometry after separation/preconcentration with nanometer-sized zirconium oxide immobilized on silica gel. Microchimica Acta 158(3-4): 227-231, 2007

On-line separation and preconcentration of inorganic arsenic and selenium species in natural water samples with CTAB-modified alkyl silica microcolumn and determination by inductively coupled plasma-optical emission spectrometry. Talanta 76(4): 772-779, 2008

Simultaneous speciation and preconcentration of ultra trace concentrations of mercury and selenium species in environmental and biological samples by hollow fiber liquid phase microextraction prior to high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. Journal of Chromatography. a 1300: 43-50, 2014

Separation, preconcentration and speciation of organic phosphorus in environmental samples. Organic phosphorus in the environment: 1-20, 2005

On-line removal of interferences via anion-exchange column separation for the determination of germanium, arsenic and selenium in biological samples by inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry 11(6): 413-420, 1996

Nanometer-sized ceria-coated silica-iron oxide for the reagentless microextraction/preconcentration of heavy metals in environmental and biological samples followed by slurry introduction to ICP-OES. Talanta 121: 127-135, 2014