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Phytoene synthase gene cloning from Citrus sinensis Osbeck cv. Cara Cara and its prokaryotic expression



Phytoene synthase gene cloning from Citrus sinensis Osbeck cv. Cara Cara and its prokaryotic expression



Agricultural Sciences in China 7(2): 148-156



Objective: The present study aimed at cloning and analysing the phytoene synthase (PSY) gene of <i>Citrus sinensis</i> Osbeck Cara Cara and expressing it in the prokaryote. Method: Using the mRNA from the fruit of Cara Cara as the template, the cDNA of PSY gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) and its sequence was analysed. The full coding sequence of PSY cDNA was cloned using PCR and further subcloned into pET28a (+).

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Accession: 021554962

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DOI: 10.1016/s1671-2927(08)60034-6


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