+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Primary structure of three fragments of proglucagon from the pancreatic islets of the daddy sculpin (Cottus scorpius)

Primary structure of three fragments of proglucagon from the pancreatic islets of the daddy sculpin (Cottus scorpius)

European Journal of Biochemistry 1641: 117-122

Three peptides isolated from the Brockmann bodies of the daddy sculpin, a teleostean fish, have been identified as fragments of one or more proglucagons. The peptide L Q D A E D S S R F D A D D T L A G E A R E L S T P K represents the NH2 terminus of proglucagon (residues 1-27), H S E G T F S N D Y S K Y L E T R R A Q D F V Q W L K N S represents glucagon and H A D G T F T S D V S S Y L N D Q A I K D F V A K L K S G K V represents the glucagon-like peptide at the COOH terminus of the precursor. The fast-atom bombardment mass spectra of the three peptides were consistent with the proposed structures and demonstrated that further posttranslational modifications of the peptides had not taken place. Sculpin glucagon is identical to anglerfish glucagon II but sculpin proglucagon (1-27) and glucagon-like peptide show stronger homology to the corresponding regions of anglefish proglucagon I than to proglucagon II. The structures of the peptides are suggestive of the action of trypsin-like and carboxypeptidase-B-like enzymes at the site of pairs of basic amino acid residues in proglucagon. The presence of a COOH-terminal lysyl group in proglucagon(1-27) may indicate, however, that the penultimate prolyl residue partially inhibits the action of the carboxypeptidase-B-like activity.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 021598899

Download citation: RISBibTeXText

PMID: 3549298

Related references

A second glucagon in the pancreatic islets of the daddy sculpin Cottus scorpius. General and Comparative Endocrinology 91(3): 281-286, 1993

Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius). Regulatory Peptides 16(3-4): 261-268, 1986

Preliminary observations on the effect of bombesin on gastric muscle in the daddy sculpin Cottus scorpius. Regulatory Peptides 46: 382, 1982

The isolation, purification and amino-acid sequence of insulin from the teleost fish Cottus scorpius (daddy sculpin). European Journal of Biochemistry 158(1): 117-123, 1986

Structural characterization of peptides derived from prosomatostatins I and II isolated from the pancreatic islets of two species of teleostean fish: the daddy sculpin and the flounder. European Journal of Biochemistry 168(3): 647-652, 1987

Habitat separation of prickly sculpin, Cottus asper, and coastrange sculpin, Cottus aleuticus, in the mainstem Smith River, Northwestern California. Copeia 199(2): 371-375, 1999

Life history and status of the shorthead sculpin cottus confusus pisces cottidae in canada and the sympatric relationship to the slimy sculpin cottus cognatus. Canadian Journal of Zoology 62(2): 306-311, 1984

Morphologically distinct populations of the shorthead sculpin cottus confusus and mottled sculpin cottus bairdi pisces cottidae near the western border of canada and the usa. Canadian Journal of Zoology 67(11): 2711-2720, 1989

Morphologically distinct populations of the shorthead sculpin, Cottus confusus, and mottled sculpin, Cottus bairdi (Pisces, Cottidae), near the western border of Canada and the United States. Canadian Journal of Zoology 6711: 2711-2720, 1989

Barriers to upstream passage by two migratory sculpins, prickly sculpin ( Cottus asper ) and coastrange sculpin ( Cottus aleuticus ), in northern Puget Sound lowland streams. Canadian Journal of Fisheries and Aquatic Sciences 71(11): 1758-1765, 2014

IGF-I in the bony fish Cottus scorpius: cDNA, expression and differential localization in brain and islets. Molecular and Cellular Endocrinology 141(1-2): 187-194, 1998

On The Agranular Cells In The Pancreatic Islet Tissue Of The Marine Teleost Cottus Scorpius. Acta Pathologica et Microbiologica Scandinavica 60: 47-54, 1964

Ultrastructure of the pancreatic islet tissue of normal and alloxan-treated Cottus scorpius. Acta Endocrinologica 39: 32-46, 1962

An intracorpusclar parasite in the blood of Cottus bubalis and Cottus scorpius. Journal of Pathology and Bacteriology Edinburgh, 18 224-227, 1913

Identification of the cells in the endocrine pancreatic tissue of the marine teleost Cottus scorpius by some silver impregnation procedures. Acta Morphologica Neerlando-Scandinavica 4: 145-152, 1961