Simultaneous kinetic-spectrophotometric determination of maltol and ethyl maltol in food samples by using chemometrics
Ni, Y.; Wang, Y.; Kokot, S.
Food Chemistry 109(2): 431-438
A fast and accurate procedure has been researched and developed for the simultaneous determination of maltol and ethyl maltol, based on their reaction with iron(III) in the presence of o-phenanthroline in sulfuric acid medium. This reaction was the basis for an indirect kinetic spectrophotometric method, which followed the development of the pink ferroin product (λmax=524nm). The kinetic data were collected in the 370-900nm range over 0-30s. The optimized method indicates that individual analytes followed Beer's law in the concentration range of 4.0-76.0mgL(-1) for both maltol and ethyl maltol. The LOD values of 1.6mgL(-1) for maltol and 1.4mgL(-1) for ethyl maltol agree well with those obtained by the alternative high performance liquid chromatography with ultraviolet detection (HPLC-UV). Three chemometrics methods, principal component regression (PCR), partial least squares (PLS) and principal component analysis-radial basis function-artificial neural networks (PC-RBF-ANN), were used to resolve the measured data with small kinetic differences between the two analytes as reflected by the development of the pink ferroin product. All three performed satisfactorily in the case of the synthetic verification samples, and in their application for the prediction of the analytes in several food products. The figures of merit for the analytes based on the multivariate models agreed well with those from the alternative HPLC-UV method involving the same samples.