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Some characteristics of haemolymph yolk protein in Pieris rapae L



Some characteristics of haemolymph yolk protein in Pieris rapae L



Korean Journal of Entomology, 181: 27-34



Physicochemical property and carbohydrate were investigated from haemolymph yolk protein of Pieris rapae L. Haemolymph yolk protein (HYP) appears as major band in upper part of gel and is present in the both male and female haemolymph of pupae. Native molecular weight of HYP was estimated to be 360,000 dalton and its pI value was determined to be pH 6.0. This protein is tetramer composed of one type of subunit which has molecular weight of 95,000 dalton. Haemolymph yolk proteins was confirmed to be conjugated protein containing carbohydrate, lipid and phosphorus. Analysis of carbohydrate conjguated in HYP shows that carbohydrate of HYP is comprised of 10.5% mannose, 24.1% .alpha.-glucose, 25.7% .beta.-glucose but less than 1% disaccharides such as lactose and maltose.

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Accession: 021768855

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