+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The developmental ability of vitrified oocytes from different mouse strains assessed by parthenogenetic activation and intracytoplasmic sperm injection



The developmental ability of vitrified oocytes from different mouse strains assessed by parthenogenetic activation and intracytoplasmic sperm injection



Journal of Reproduction and Development 53(6): 1199-1206



Assessment of the developmental ability of oocytes following freezing and thawing is an important step for optimizing oocyte cryopreservation techniques. However, the in vitro fertilization of frozen-thawed mouse oocytes is often inefficient because of incomplete capacitation of spermatozoa in the absence of surrounding cumulus cells. This study was undertaken to determine whether the oocyte cryopreservation efficiency of different strains of mice could be assessed from the development of oocytes following parthenogenetic activation and intracytoplasmic sperm injection (ICSI). Oocytes were collected from hybrid (C57BL/6 x DBA/2) F1 or inbred (C57BL/6J, C3H/HeN, DBA/2J and BALB/cA) strains and were vitrified in a solution containing ethylene glycol, DMSO, Ficoll and sucrose. In the first series of experiments, oocytes were activated parthenogenetically by Sr(2+) treatment after warming. The oocytes from the inbred strains, but not those of the F1 hybrid, were diploidized by cytochalasin treatment to obtain a sufficient number of blastocysts. In all strains tested, parthenogenetic embryos derived from vitrified oocytes developed into blastocysts at rates between 23 and 68%. In the second series of experiments, vitrified oocytes from each strain were injected with homologous spermatozoa after warming. Normal offspring were obtained from all strains at rates between 5 and 26% per embryo transferred. Thus, the feasibility of oocyte cryopreservation protocols can be assessed easily by in vitro development of parthenogenetic embryos or by in vivo development of ICSI embryos. Moreover, the oocytes of these four major inbred strains of mice can be cryopreserved safely for production of offspring.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 021907232

Download citation: RISBibTeXText

PMID: 17827873

DOI: 10.1262/jrd.19058


Related references

Developmental capacity of Antarctic minke whale ( Balaenoptera bonaerensis ) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection. Zygote 14(2): 89-95, 2006

In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection. Theriogenology 75(9): 1652-1660, 2011

Use of mouse oocytes to evaluate the ability of human sperm to activate oocytes after failure of activation by intracytoplasmic sperm injection. Zygote 12(2): 111-116, 2004

Microtubule organisation, pronuclear formation and embryonic development of mouse oocytes after intracytoplasmic sperm injection or parthenogenetic activation and then slow-freezing with 1, 2-propanediol. Reproduction, Fertility, and Development 25(4): 609-616, 2013

Oocyte activation and parthenogenetic development of bovine oocytes following intracytoplasmic sperm injection. Zygote 7(3): 233-237, 1999

In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fertility and Sterility 87(1): 77-82, 2006

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection. Journal of Assisted Reproduction and Genetics 33(12): 1685-1690, 2016

Birth of a healthy male infant after transfer of vitrified-warmed blastocysts derived from intracytoplasmic sperm injection with vitrified-warmed oocytes and frozen-thawed spermatozoa. Journal of Assisted Reproduction and Genetics 26(8): 451-453, 2010

Activation of mouse oocytes following intracytoplasmic injection of chicken sperm extract. Reproduction in Domestic Animals 38(5): 401-404, 2003

Pregnancy outcomes and maternal and perinatal complications of pregnancies following in vitro fertilization/intracytoplasmic sperm injection using own oocytes, donor oocytes, and vitrified embryos: A prospective follow-up study. Journal of Human Reproductive Sciences 9(4): 241-249, 2017

Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage. Cryobiology 75: 52-59, 2017

Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into vitrified human oocytes. Human Fertility 11(1): 49-51, 2008

Effects of different activation regimens on pronuclear formation and developmental competence of in vitro-matured porcine oocytes after intracytoplasmic sperm injection. Reproduction in Domestic Animals 47(4): 609-614, 2012

Development into non-parthenogenetic blastocysts from metaphase I bovine oocytes fertilized by intracytoplasmic sperm injection. Theriogenology 57(1): 755, January 2, 2002

Effects of age and equine follicle-stimulating hormone (eFSH) on collection and viability of equine oocytes assessed by morphology and developmental competency after intracytoplasmic sperm injection (ICSI). Reproduction, Fertility, and Development 21(4): 615-623, 2009