+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Two transfer RNA sequences abut the large ribosomal RNA gene in Tetrahymena mitochondrial DNA: tRNA leu (anticodon UAA) and tRNA met (anticodon CAU)



Two transfer RNA sequences abut the large ribosomal RNA gene in Tetrahymena mitochondrial DNA: tRNA leu (anticodon UAA) and tRNA met (anticodon CAU)



Current Genetics 114: 327-330



The sequence of a 1,427 base pair restriction fragment, HaeIII fragment 6, of the ciliate protozoan Tetrahymena mitochondrial DNA, is presented. The first 780 nucleotide sequence aligns well with the terminal segment of the large rDNA sequence of Paramecium mitochondria. Immediately abutting this rDNA termination sequence, a tRNA sequence was found with anticodon UAA for leucine. The derived tRNA sequence is 81 bases long without the 3' CCA end, has a high G + C content of 48.1%, and can be folded into a normal cloverleaf structure with mostly conserved bases and normal stems and loops. The tRNA sequence found at an analogous position of the Paramecium mitochondrial DNA is tRNAtyr. Following a highly A + T rich sequence of 300 base pairs, another tRNA-like sequence is present; this putative tRNA has only 67 bases with anticodon CAT (Met) and forms standard aminoacyl, anticodon and T .psi. C stems with a conventional T .psi. C loop. However, the DHU loop and stem are unusually short and irregualr; the base at position 8 is G instead of T; and the base following the anticodon, which is normally a purine, is T. The significance of these tRNA structures is discussed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 022043296

Download citation: RISBibTeXText

PMID: 3129201


Related references

Two transfer rna sequences abut the large ribosomal rna gene in tetrahymena mitochondrial dna leucine transfer rna anticodon uaa and methionine transfer rna anticodon cau. Current Genetics 11(4): 327-330, 1987

Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry 11(2): 301-308, 1972

The tRNA anticodon TTU gene and its upstream sequence coding for a homolog of the E coli large ribosome-subunit protein L14 in the Tetrahymena mitochondrial genome. Nucleic Acids Research 17.2: 803, 1989

Anticodon-anticodon interaction induces conformational changes in tRNA: yeast tRNAAsp, a model for tRNA-mRNA recognition. Proceedings of the National Academy of Sciences of the United States of America 83(4): 932-936, 1986

Orientation of the tRNA anticodon in the ribosomal P-site: quantitative footprinting with U33-modified, anticodon stem and loop domains. Rna 5(9): 1191-1199, 1999

Studies on anticodon-anticodon interactions: hemi-protonation of cytosines induces self-pairing through the GCC anticodon of E. coli tRNA-Gly. Journal of Biomolecular Structure and Dynamics 4(2): 193-203, 1986

Effect of threonylcarbamoyl modification (t6A) in yeast tRNA Arg III on codon-anticodon and anticodon-anticodon interactions. A thermodynamic and kinetic evaluation. European Journal of Biochemistry 116(1): 207-213, 1981

Rat liver mitochondrial lysine tRNA (anticodon U*UU) contains a rudimentary D-arm and 2 hypermodified nucleotides in its anticodon loop. Biochemical and Biophysical Research Communications 103(2): 739-744, 1981

The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena. Genes & Development 10(22): 2870-2880, 1996

TRNA structure and ribosomal function. II. Interaction between anticodon helix and other tRNA mutations. Journal of Molecular Biology 235(5): 1395-1405, 1994

Dinucleotide codon-anticodon interaction as a minimum requirement for ribosomal aa-tRNA binding: stabilisation by viomycin of aa-tRNA in the A site. Nucleic Acids Research 8(23): 5813-5824, 1980

Dinucleotide codon-anticodon interaction as a minimum requirement for ribosomal as-tRna binding: stabilisation by viomycin of aa-tRna in the A site. Nucleic Acids Research 8(23): 5813-5824, 1980

Unconventional structure of tRNA(Lys)SUU anticodon explains tRNA's role in bacterial and mammalian ribosomal frameshifting and primer selection by HIV-1. Rna 3(4): 420-428, 1997

Mitochondrial tRNA diseases: defect of modification in anticodon in mutant tRNA molecules. Tanpakushitsu Kakusan Koso. Protein Nucleic Acid Enzyme 48(4 Suppl): 493-500, 2003

Chemical synthesis of the 5-taurinomethyl(-2-thio)uridine modified anticodon arm of the human mitochondrial tRNA(Leu(UUR)) and tRNA(Lys). Rna 20(6): 938-947, 2014