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Using a commercial DNA extraction kit to obtain RNA for RT-PCR from starchy rice endosperm


Biotechnology Journal 3(3): 360-363
Using a commercial DNA extraction kit to obtain RNA for RT-PCR from starchy rice endosperm
The extraction of RNA from a starchy plant material, such as many common food grains, is difficult, and especially so from the mature endosperm of rice. Most commercial RNA kits are not suitable for starchy materials. Traditional RNA extraction procedures, in addition to being laborious and time consuming, leave hazardous organic wastes that result in expensive disposal costs.


Accession: 022069620

PMID: 18228540

DOI: 10.1002/biot.200700225



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