EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Using a commercial DNA extraction kit to obtain RNA for RT-PCR from starchy rice endosperm






Biotechnology Journal 3(3): 360-363

Using a commercial DNA extraction kit to obtain RNA for RT-PCR from starchy rice endosperm

The extraction of RNA from a starchy plant material, such as many common food grains, is difficult, and especially so from the mature endosperm of rice. Most commercial RNA kits are not suitable for starchy materials. Traditional RNA extraction procedures, in addition to being laborious and time consuming, leave hazardous organic wastes that result in expensive disposal costs.

(PDF same-day service: $19.90)

Accession: 022069620

PMID: 18228540

DOI: 10.1002/biot.200700225



Related references

Glycoproteins in rice starchy endosperm. Agricultural & Biological Chemistry 54(10): 2731-2732, 1990

Diffusivity of starchy endosperm and bran of fresh and re wetted rice. Journal of Food Science 45(2): 356-361, 1980

Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm. Journal of Experimental Botany 59(15): 4233-4245, 2008

Studies on the programmed cell death in rice during starchy endosperm development. Agricultural Sciences in China 3(9): 663-670, 2004

Formation of protein bodies in the starchy endosperm of rice oryza sativa a reinvestigation. Annals of Botany (London) 45(5): 503-510, 1980

Ultrastructure of the mature ungerminated rice oryza sativa caryopsis the starchy endosperm. American Journal of Botany 65(6): 684-691, 1978

Spatial and temporal progress of programmed cell death in the developing starchy endosperm of rice. Planta 237(5): 1393-1400, 2013

Degeneration of nucleus and ultracytochemical localization of hydrolytic enzymes during starchy endosperm development in rice. Acta Agronomica Sinica 32(2): 298-300, 2006

Ultrastructure of compound starch granules and protein bodies of starchy endosperm cell in rice. Agricultural Chemistry & Biotechnology 39(5): 379-383, 1996

The starchy endosperm denucleation by a process of programmed cell death during rice grain development. Shi Yan Sheng Wu Xue Bao 37(1): 34-44, 2004