+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of the effects of Percoll and PureSperm on the common marmoset (Callithrix jacchus) semen

Comparison of the effects of Percoll and PureSperm on the common marmoset (Callithrix jacchus) semen

Journal of Medical Primatology 34(2): 86-90

Density gradient centrifugation is a widely used technique for the separation of motile from non-motile sperm, for the removal of contaminating agents such as bacteria and viruses, and for the removal of seminal plasma or cryoprotectant. In the choice of a density gradient medium for a new species, it is important to perform toxicity tests. The present study was carried out to evaluate the potential toxic effects of two silica-based density gradient products (Percoll and PureSperm), on the sperm of the common marmoset. We assessed two different batches of Percoll (polyvinylpyrrolidone (PVP)-coated colloidal silica particles) and one of PureSperm (saline-coated colloidal silica suspension) by means of a computer-aided sperm analysis on semen collected by vibrostimulation. The results showed that although some of the sperm patterns of movement and viability changed significantly over time, and provide a first description of marmoset sperm motility changes under capacitating conditions, there was no significant difference in the sperm treated with Percoll or PureSperm in comparison with the control. We conclude that simple exposure to either of these products does not have a negative effect on viability or motility of marmoset sperm.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 022247853

Download citation: RISBibTeXText

PMID: 15860114

DOI: 10.1111/j.1600-0684.2005.00095.x

Related references

Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted-ear marmoset (Callithrix penicillata). Journal of Medical Primatology 48(1): 32-42, 2019

Semen characteristics of captive common marmoset (Callithrix jacchus): a comparison of a German with a Brazilian colony. Journal of Medical Primatology 43(4): 225-230, 2014

Collection and analysis of semen from the common marmoset (Callithrix jacchus). Journal of Andrology 12(3): 214-220, 1991

Protozoan and helminth parasitism in common marmoset Callithrix jacchus Parasitismo por helmintos e protozoarios no sagui comum Callithrix jacchus. Unknown, 1997

Diurnal variation in common marmoset Callithrix jacchus sociosexual behaviors Variacao diurna em comportamentos socio-sexuais do sagui, Callithrix jacchus. Unknown, 1997

Influence of semen collection method on ejaculate characteristics in the common marmoset, Callithrix jacchus. Journal Of Andrology. 17(2): 164-172, 1996

A non-invasive method of weighing amongst a group of semi free common marmoset Callithrix jacchus Eine nichtinvasive Methode der Gewichtserfassung an einer Gruppe semifreilebender Weissbueschelaffen Callithrix jacchus. Zoologische Garten. Dezember; 736: 422-425, 2003

The intersexual agonistic behavior related to food access in common marmoset Callithrix jacchus pairs O comportamento agonista intersexos em funcao do acesso ao alimento em pares de saguis Callithrix jacchus. Unknown, 1997

Hybridization effects and genetic diversity of the common and black-tufted marmoset (Callithrix jacchus and Callithrix penicillata) mitochondrial control region. American Journal of Physical Anthropology 155(4): 522-536, 2014

Playful interactions in family groups of the common marmoset callithrix jacchus jacchus. Animal Behaviour 30(3): 886-900, 1982

Connections of the primary auditory cortex in the common marmoset, Callithrix jacchus jacchus. Journal of Comparative Neurology 269(2): 235-248, 1988

An experimental analysis of social interaction in the common marmoset callithrix jacchus jacchus. International Journal of Primatology 5(6): 591-607, 1984

The pair bond of the common marmoset callithrix jacchus jacchus an experimental investigation. Animal Behaviour 31(3): 651-658, 1983

The fertility of daughters in common marmoset callithrix jacchus jacchus family groups. Primates 30(3): 423-432, 1989

Frequency representation in auditory cortex of the common marmoset (Callithrix jacchus jacchus). Journal of Comparative Neurology 252(2): 175-185, 1986