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Derivation of functional insulin-producing cell lines from primary mouse embryo culture

Derivation of functional insulin-producing cell lines from primary mouse embryo culture

Stem Cell Research 2(1): 29-40

ISSN/ISBN: 1873-5061

PMID: 19383407

DOI: 10.1016/j.scr.2008.07.004

We have previously described the derivation of insulin-producing cell lines from mouse embryonic stem cells (mESCs) by differentiation of an intermediate lineage-restricted E-RoSH cell line through nutrient depletion in the presence of nicotinamide followed by limiting dilution. Here we investigated whether insulin-producing cell lines could be similarly derived directly from mouse embryo cells or tissues.

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Accession: 022348228

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